Monkey Focal Adhesion Kinase ELISA Kit | FAK elisa kit

Monkey Focal Adhesion Kinase ELISA Kit

Cat. Num. AAA753259

• Gene Names: PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• Reactivity: Monkey
• Synonyms: Focal Adhesion Kinase; Monkey Focal Adhesion Kinase ELISA Kit; FAK elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FAK elisa kit

Principle of the assay: FAK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FAK antibody and an FAK-HRP conjugate. The assay sample and buffer are incubated together with FAK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAK concentration since FAK from samples and FAK-HRP conjugate compete for the anti-FAK antibody binding site. Since the number of sites is limited, as more sites are occupied by FAK from the sample, fewer sites are left to bind FAK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 439875
• NCBI GeneID: 5747
• Molecular Weight: 39,874 Da
• NCBI Official Full Name: focal adhesion kinase
• NCBI Official Synonym Full Names: protein tyrosine kinase 2
• NCBI Official Symbol: PTK2
• NCBI Official Synonym Symbols: FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
• NCBI Protein Information: focal adhesion kinase 1; FADK 1; PTK2 protein tyrosine kinase 2; FAK-related non-kinase polypeptide; focal adhesion kinase isoform FAK-Del33; focal adhesion kinase-related nonkinase; protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71
• UniProt Protein Name: Focal adhesion kinase 1
• Protein Family: Focal adhesion kinase
• UniProt Gene Name: PTK2
• UniProt Synonym Gene Names: FAK; FAK1; FADK 1; FRNK; PPP1R71
• UniProt Entry Name: FAK1_HUMAN

NCBI Description

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq, Dec 2010]

Uniprot Description

FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; Kinase, protein; TK group; Fak family

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; focal adhesion; lamellipodium; apical plasma membrane; cytoplasm; plasma membrane; stress fiber; microtubule organizing center; cell cortex; nucleus; cytosol

Molecular Function: JUN kinase binding; signal transducer activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; SH2 domain binding; actin binding; protein kinase binding; ATP binding; receptor binding; protein kinase activity

Biological Process: heart morphogenesis; axon guidance; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; establishment of nucleus localization; apoptosis; protein amino acid autophosphorylation; neuron migration; cell motility involved in cell locomotion; negative regulation of synaptogenesis; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; vasculogenesis; placenta development; cell structure disassembly during apoptosis; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; regulation of osteoblast differentiation; central nervous system neuron axonogenesis; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; embryonic development; establishment of cell polarity; positive regulation of protein kinase activity; regulation of focal adhesion formation; innate immune response; endothelial cell migration; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; blood coagulation; vascular endothelial growth factor receptor signaling pathway; negative regulation of apoptosis; regulation of cytoskeleton organization and biogenesis; positive regulation of cell migration