Mouse Senataxin Monoclonal Antibody | anti-SETX antibody

Senataxin (Probable Helicase Senataxin, SETX, Amyotrophic Lateral Sclerosis 4 Protein, ALS4, AOA2, bA479K20.2, KIAA0625, SCAR1, SEN1 Homolog) (MaxLight 750)

Cat. Num. AAA6235263

• Gene Names: SETX; ALS4; AOA2; Sen1; SCAN2; SCAR1; bA479K20.2
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Senataxin; Monoclonal Antibody; Senataxin (Probable Helicase Senataxin; SETX; Amyotrophic Lateral Sclerosis 4 Protein; ALS4; AOA2; bA479K20.2; KIAA0625; SCAR1; SEN1 Homolog) (MaxLight 750); EC=3.6.4.-; anti-SETX antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A9
• Specificity: Recognizes human SETX. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4.
• Sequence Length: 11101

• Applicable Applications for anti-SETX antibody: FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant protein corresponding to aa2579-2676 from human SETX with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: VVHQDLSHIQQPAAVVAALSSHKPPVRGEPPAASPEASTCQSKCDDPEEELCHRREARAFSEGEQEKCGSETHHTRRNSRWDKRTLEQEDSSSKKRKL*
• Conjugate: MaxLight750
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged SETX is 0.3ng/ml using MBS641490 as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of SETX expression in PC-12 using 133213)

Product Categories/Family for anti-SETX antibody

Antibodies; DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 1519311427
• NCBI GeneID: 23064
• NCBI Accession #: NM_015046.7
• NCBI GenBank Nucleotide #: NM_015046
• UniProt Accession #: Q7Z333
• NCBI Official Full Name: Homo sapiens senataxin (SETX), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: senataxin
• NCBI Official Symbol: SETX
• NCBI Official Synonym Symbols: ALS4; AOA2; Sen1; SCAN2; SCAR1; bA479K20.2
• NCBI Protein Information: probable helicase senataxin
• UniProt Protein Name: Probable helicase senataxin
• Protein Family: Probable helicase senataxin
• UniProt Gene Name: SETX

NCBI Description

This gene encodes a protein named for its homology to the Sen1p protein of fungi which has RNA helicase activity encoded by a domain at the C-terminal end of the protein. The protein encoded by this gene contains a DNA/RNA helicase domain at its C-terminal end which suggests that it may be involved in both DNA and RNA processing. Mutations in this gene have been associated with ataxia-ocular apraxia-2 (AOA2) and an autosomal dominant form of juvenile amyotrophic lateral sclerosis (ALS4). [provided by RefSeq, Jul 2008]

Uniprot Description

senataxin: Probable helicase, which may be involved in RNA maturation. Involved in DNA double-strand breaks damage response generated by oxidative stress. Defects in SETX are the cause of spinocerebellar ataxia autosomal recessive type 1 (SCAR1); also known as ataxia-ocular apraxia 2. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR1 is an autosomal recessive form associated with peripheral neuropathy and elevated serum alpha- fetoprotein, immunoglobulins and, less commonly, creatine kinase levels. Some SCAR1 patients manifest oculomotor apraxia. Defects in SETX are a cause of amyotrophic lateral sclerosis type 4 (ALS4). ALS4 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms. ALS4 is a childhood- or adolescent- onset form characterized by slow disease progression and the sparing of bulbar and respiratory muscles. Belongs to the DNA2/NAM7 helicase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase; Nucleolus

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: axon; chromosome, telomeric region; cytoplasm; growth cone; intercellular bridge; nuclear body; nuclear chromosome; nucleolus; nucleoplasm; nucleus

Molecular Function: ATP binding; DNA binding; DNA helicase activity; identical protein binding; protein binding

Biological Process: cellular response to DNA damage stimulus; cellular response to fibroblast growth factor stimulus; cellular response to hydrogen peroxide; cellular response to oxidative stress; cellular response to retinoic acid; circadian rhythm; DNA duplex unwinding; DNA recombination; DNA-templated transcription, termination; double-strand break repair; fibroblast growth factor receptor signaling pathway; MAPK cascade; mRNA splice site selection; negative regulation of apoptosis; positive regulation of DNA-templated transcription, initiation; positive regulation of neuron projection development; positive regulation of RNA splicing; positive regulation of termination of RNA polymerase II transcription, poly(A)-coupled; positive regulation of transcription from RNA polymerase II promoter; protein kinase B signaling; RNA processing; spermatogenesis; termination of RNA polymerase II transcription

Disease: Amyotrophic Lateral Sclerosis 4, Juvenile; Spinocerebellar Ataxia, Autosomal Recessive 1