Mouse fatty acid binding protein 4, adipocyte ELISA Kit | FABP4 elisa kit

Mouse Fatty acid-binding protein, adipocyte, FABP4 ELISA Kit

Cat. Num. AAA925921

• Gene Names: Fabp4; Ap2; Lbpl; 422/aP2; ALBP/Ap2
• Reactivity: Mouse
• Synonyms: fatty acid binding protein 4; adipocyte; Mouse Fatty acid-binding protein; FABP4 ELISA Kit; adipocyte (FABP4) ELISA kit; A-FABP; aP2; ; FABP4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse FABP4. No significant cross-reactivity or interference between mouse FABP4 and analogues was observed.
• Sequence Length: 132

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml
• Sensitivity: 7.81 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Related Product Information for FABP4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FABP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FABP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FABP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FABP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 14149635
• NCBI GeneID: 11770
• NCBI Accession #: NP_077717.1
• NCBI GenBank Nucleotide #: NM_024406.2
• UniProt Accession #: P04117
• Molecular Weight: 14,650 Da
• NCBI Official Full Name: fatty acid-binding protein, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: Fabp4
• NCBI Official Synonym Symbols: Ap2; Lbpl; 422/aP2; ALBP/Ap2
• NCBI Protein Information: fatty acid-binding protein, adipocyte; P15; ALBP; AFABP; A-FABP; protein 422; P2 adipocyte protein; adipocyte protein aP2; myelin P2 protein homolog; 3T3-L1 lipid-binding protein; fatty acid-binding protein 4; adipocyte lipid-binding protein; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: Fabp4
• UniProt Synonym Gene Names: Ap2; ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_MOUSE

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: transporter activity; lipid binding

Biological Process: cholesterol homeostasis; transport; positive regulation of cell proliferation; white fat cell differentiation; brown fat cell differentiation; cytokine production; negative regulation of protein kinase activity; fatty acid metabolic process; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response