Bovine Fatty Acid Binding Protein 4, Adipocyte ELISA Kit | FABP4 elisa kit

Bovine Fatty Acid Binding Protein 4, Adipocyte ELISA Kit

Cat. Num. AAA736377

• Gene Names: FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104
• Reactivity: Bovine
• Synonyms: Fatty Acid Binding Protein 4; Adipocyte; Bovine Fatty Acid Binding Protein 4; Adipocyte ELISA Kit; FABP4 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Standard Curve/Testing Data

Related Product Information for FABP4 elisa kit

Intended Uses: This FABP4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine FABP4. This ELISA kit for research use only!

Principle of the Assay: FABP4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FABP4 antibody and an FABP4-HRP conjugate. The assay sample and buffer are incubated together with FABP4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FABP4 concentration since FABP4 from samples and FABP4-HRP conjugate compete for the anti-FABP4 antibody binding site. Since the number of sites is limited, as more sites are occupied by FABP4 from the sample, fewer sites are left to bind FABP4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP4 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119607498
• NCBI GeneID: 2167
• Molecular Weight: 14,719 Da
• NCBI Official Full Name: fatty acid binding protein 4, adipocyte
• NCBI Official Synonym Full Names: fatty acid binding protein 4, adipocyte
• NCBI Official Symbol: FABP4
• NCBI Official Synonym Symbols: aP2; ALBP; AFABP; A-FABP; HEL-S-104
• NCBI Protein Information: fatty acid-binding protein, adipocyte; fatty acid-binding protein 4; adipocyte lipid-binding protein; epididymis secretory protein Li 104; adipocyte-type fatty acid-binding protein
• UniProt Protein Name: Fatty acid-binding protein, adipocyte
• Protein Family: Fatty acid-binding protein
• UniProt Gene Name: FABP4
• UniProt Synonym Gene Names: ALBP; A-FABP; AFABP
• UniProt Entry Name: FABP4_HUMAN

NCBI Description

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

FABP4: a lipid transport protein that is the predominant fatty acid binding protein found in adipocytes. Also expressed in macrophages. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. Homodimer. Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Interacts with PPARG. Monomer. FABP4 knockout mice fed a high-fat and high-calorie diet become obese but develop neither insulin resistance nor diabetes, suggesting that this protein might be a link between obesity and insulin resistance and diabetes. Mice deficient in both FABP4 and ApoE show protection against atherosclerosis when compared with mice deficient only in ApoE. Mice carrying a FABP4 genetic variant exhibit both reduced FABP4 expression and a reduced potential for developing type 2 diabetes and coronary heart disease. A related study in humans indicated a similar pattern, suggesting that FABP4 may be a potential therapeutic target in the treatment of these disorders. Belongs to the calycin superfamily,fatty-acid binding protein (FABP) family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 8q21

Cellular Component: cytoplasm; lipid particle; nucleus

Molecular Function: transporter activity; fatty acid binding

Biological Process: cholesterol homeostasis; transport; white fat cell differentiation; triacylglycerol catabolic process; brown fat cell differentiation; cytokine production; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; positive regulation of inflammatory response