Rabbit MSH5 Polyclonal Antibody | anti-MSH5 antibody

Anti-MSH5 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100840

• Gene Names: MSH5; G7; NG23; MUTSH5
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MSH5; Polyclonal Antibody; Anti-MSH5 Antibody; Rabbit Polyclonal; MSH5 Antibody; Rabbit PAb; Antigen Affinity Purified; mutS homolog 5; Anti-DAAP-21F2.10-001 Antibody; Anti-G7 Antibody; Anti-MUTSH5 Antibody; Anti-NG23 Antibody; anti-MSH5 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MSH5
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MSH5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the human MSH5
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the human MSH5, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MSH5 rabbit polyclonal antibody at 1:500 dilutionLane A: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:93 kDaObserved band size:93 kDa)

Related Product Information for anti-MSH5 antibody

MSH5 (MutSHomolog 5) is localized into the mitochondria of germ and somatic cells. This protein binds to mtDNA and interacts with the Twinkle helicase and the DNA polymerase gamma. hMSH5 stimulates mtDNA repair in response to DNA damage induced by oxidative stress. Since MSH5 encodes a protein homologous to DNA mismatch recognition proteins, mismatch repair genes are frequently mutated in cancers cells and, like mismatch repair genes, MSH5 is highly conserved from yeast to human, this observation suggests novel mechanisms of chemotherapeutic drug resistance that may occur in certain human cancer patients. The human MSH5 gene consists of 25 exons and spans at least 12 kb of genomic DNA, while the C. elegans gene comprises 17 exons distributed over at least 5.8 kb.

NCBI and Uniprot Product Information

• NCBI GI #: 4529899
• NCBI GeneID: 4439
• UniProt Accession #: O43196; O60586; Q5BLU9; Q5SSR1; Q8IW44; Q9BQC7; B0V033; B0V034
• Molecular Weight: 92,875 Da
• NCBI Official Full Name: MutSH5
• NCBI Official Synonym Full Names: mutS homolog 5
• NCBI Official Symbol: MSH5
• NCBI Official Synonym Symbols: G7; NG23; MUTSH5
• NCBI Protein Information: mutS protein homolog 5
• UniProt Protein Name: MutS protein homolog 5
• Protein Family: MutS protein
• UniProt Gene Name: MSH5
• UniProt Synonym Gene Names: hMSH5
• UniProt Entry Name: MSH5_HUMAN

NCBI Description

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]

Uniprot Description

MSH5: Involved in meiotic recombination. Facilitate crossovers between homologs during meiosis. Belongs to the DNA mismatch repair MutS family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: synaptonemal complex

Molecular Function: DNA-dependent ATPase activity; protein binding; damaged DNA binding; ATP binding; mismatched DNA binding

Biological Process: homologous chromosome segregation; meiotic recombination; chiasma formation; meiotic mismatch repair