Human Proteinase-activated receptor 1 ELISA Kit | F2R elisa kit

Human Proteinase-activated receptor 1 ELISA Kit

Cat. Num. AAA9426146

• Gene Names: F2R; TR; HTR; CF2R; PAR1; PAR-1
• Reactivity: Human
• Synonyms: Proteinase-activated receptor 1; Human Proteinase-activated receptor 1 ELISA Kit; PAR-1; Coagulation factor II receptor; Thrombin receptor; F2R; CF2R; PAR1; TR; F2R elisa kit

• Reactivity: Human
• Specificity: This assay recognizes recombinant and natural human PAR-1. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma, tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 0.31 ng/mL -20.0 ng/mL
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F2R elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human proteinase-activated receptor 1,PAR-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PAR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PAR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PAR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PAR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 913402869
• NCBI GeneID: 2149
• NCBI Accession #: NP_001298242.1
• NCBI GenBank Nucleotide #: NM_001311313.1
• UniProt Accession #: P25116; Q53XV0; Q96RF7; Q9BUN4
• Molecular Weight: 47,441 Da
• NCBI Official Full Name: proteinase-activated receptor 1 isoform 2
• NCBI Official Synonym Full Names: coagulation factor II thrombin receptor
• NCBI Official Symbol: F2R
• NCBI Official Synonym Symbols: TR; HTR; CF2R; PAR1; PAR-1
• NCBI Protein Information: proteinase-activated receptor 1
• UniProt Protein Name: Proteinase-activated receptor 1
• Protein Family: Proteinase-activated receptor
• UniProt Gene Name: F2R
• UniProt Synonym Gene Names: CF2R; PAR1; TR; PAR-1

NCBI Description

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

Uniprot Description

PAR1: a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.

Protein type: Cell development/differentiation; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: caveola; cell surface; early endosome; extracellular region; Golgi apparatus; integral to plasma membrane; late endosome; neuromuscular junction; plasma membrane; postsynaptic membrane

Molecular Function: G-protein alpha-subunit binding; G-protein beta-subunit binding; G-protein coupled receptor activity; protein binding; receptor binding; thrombin receptor activity

Biological Process: activation of MAPKK activity; anatomical structure morphogenesis; blood coagulation; caspase activation; connective tissue replacement during inflammatory response; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); establishment of synaptic specificity at neuromuscular junction; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); homeostasis of number of cells within a tissue; inflammatory response; negative regulation of cell proliferation; negative regulation of glomerular filtration; negative regulation of neuron apoptosis; platelet activation; platelet dense granule organization and biogenesis; positive regulation of blood coagulation; positive regulation of calcium ion transport; positive regulation of caspase activity; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of Rho protein signal transduction; positive regulation of smooth muscle contraction; positive regulation of transcription, DNA-dependent; positive regulation of vasoconstriction; protein kinase C activation; regulation of blood coagulation; regulation of interleukin-1 beta production; regulation of sensory perception of pain; release of sequestered calcium ion into cytosol; response to lipopolysaccharide; response to wounding; STAT protein nuclear translocation; tyrosine phosphorylation of STAT protein