Goat Free haemoglobin ELISA Kit | F-Hb elisa kit
Goat Free haemoglobin ELISA Kit
Reactivity
Goat
Synonyms
Free haemoglobin; Goat Free haemoglobin ELISA Kit; F-Hb elisa kit
Reactivity
Goat
Specificity
This assay has high sensitivity and excellent specificity for detection of FHB. No significant cross-reactivity or interference between FHB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FHB and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ug/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for F-Hb elisa kit
Intended Uses: This FHB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat FHB. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: FHB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FHB antibody and an FHB-HRP conjugate. The assay sample and buffer are incubated together with FHB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FHB concentration since FHB from samples and FHB-HRP conjugate compete for the anti-FHB antibody binding site. Since the number of sites is limited, as more sites are occupied by FHB from the sample, fewer sites are left to bind FHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FHB concentration in each sample is interpolated from this standard curve.
Principle of the Assay: FHB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FHB antibody and an FHB-HRP conjugate. The assay sample and buffer are incubated together with FHB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FHB concentration since FHB from samples and FHB-HRP conjugate compete for the anti-FHB antibody binding site. Since the number of sites is limited, as more sites are occupied by FHB from the sample, fewer sites are left to bind FHB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FHB concentration in each sample is interpolated from this standard curve.
Product Categories/Family for F-Hb elisa kit
