Human Ezrin ELISA Kit | EZR elisa kit

Human Ezrin ELISA Kit

Cat. Num. AAA760259

• Gene Names: EZR; CVL; CVIL; VIL2; HEL-S-105
• Reactivity: Human
• Synonyms: Ezrin; Human Ezrin ELISA Kit; EZR/Ezrin/p81/Cytovillin/Villin-2/VIL2; EZR elisa kit

• Reactivity: Human
• Sequence Length: 586

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for EZR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human EZRantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human EZR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human EZR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman EZRcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 161702986
• NCBI GeneID: 7430
• NCBI Accession #: NP_001104547.1
• NCBI GenBank Nucleotide #: NM_001111077.1
• Molecular Weight: 69,413 Da
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2; HEL-S-105
• NCBI Protein Information: ezrin
• UniProt Protein Name: Ezrin
• Protein Family: Ezrin
• UniProt Gene Name: EZR
• UniProt Synonym Gene Names: VIL2
• UniProt Entry Name: EZRI_HUMAN

NCBI Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: actin cytoskeleton; actin filament; apical part of cell; apical plasma membrane; basolateral plasma membrane; brush border; cell projection; cortical cytoskeleton; cytoplasm; cytosol; endosome; extracellular space; extrinsic to membrane; filopodium; focal adhesion; immunological synapse; membrane; microvillus; microvillus membrane; myelin sheath; nucleolus; perinuclear region of cytoplasm; plasma membrane; ruffle; T-tubule; uropod; vesicle

Molecular Function: actin binding; actin filament binding; ATPase binding; cell adhesion molecule binding; microtubule binding; protein binding; protein domain specific binding

Biological Process: actin cytoskeleton reorganization; actin filament bundle formation; astral microtubule organization and biogenesis; axon guidance; cortical microtubule organization and biogenesis; cytoskeletal anchoring; D-glucose absorption; establishment of centrosome localization; establishment of epithelial cell polarity; filopodium formation; gland morphogenesis; leukocyte adhesion; membrane to membrane docking; microvillus biogenesis; negative regulation of T cell receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; phosphoinositide-mediated signaling; positive regulation of multicellular organism growth; positive regulation of protein secretion; receptor internalization; regulation of actin cytoskeleton organization and biogenesis; regulation of cell shape; regulation of cell size; regulation of microvillus length