Rabbit anti-Human AP3M1 Polyclonal Antibody | anti-AP3M1 antibody

AP3M1, CT (AP3M1, AP-3 complex subunit mu-1, AP-3 adapter complex mu3A subunit, Adapter-related protein complex 3 mu-1 subunit, Mu-adaptin 3A, Mu3A-adaptin) (FITC)

Cat. Num. AAA6274118

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP3M1; Polyclonal Antibody; CT (AP3M1; AP-3 complex subunit mu-1; AP-3 adapter complex mu3A subunit; Adapter-related protein complex 3 mu-1 subunit; Mu-adaptin 3A; Mu3A-adaptin) (FITC); Mu3A-adaptin; anti-AP3M1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AP3M1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: AP3M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the C-terminal region of human AP3M1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AP3M1 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the AP3M1 antibody detected the AP3M1 protein (arrow).)

Related Product Information for anti-AP3M1 antibody

The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. AP-3 facilitates the budding of vesicles from the Golgi membrane and may be directly involved in protein sorting to the endosomal/lysosomal system. AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. Alternatively spliced transcript variants encoding the same protein have been observed.

Product Categories/Family for anti-AP3M1 antibody

Antibodies; Adapter Proteins

References

Hashimoto, R., et al. Neurosci. Res. 65(1):113-115(2009). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Madrid, R., et al. EMBO J. 20(24):7008-7021(2001). Drake, M.T., et al. Mol. Biol. Cell 11(11):3723-3736(2000). Dell'Angelica, E.C., et al. Mol. Cell 3(1):11-21(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 6912240
• NCBI GeneID: 26985
• NCBI Accession #: NP_036227.1
• NCBI GenBank Nucleotide #: NM_012095.4
• UniProt Accession #: Q9Y2T2; Q5JQ12; Q9H5L2
• Molecular Weight: 46,939 Da
• NCBI Official Full Name: AP-3 complex subunit mu-1
• NCBI Official Synonym Full Names: adaptor-related protein complex 3, mu 1 subunit
• NCBI Official Symbol: AP3M1
• NCBI Protein Information: AP-3 complex subunit mu-1; mu3A-adaptin; mu-adaptin 3A; AP-3 adapter complex mu3A subunit; AP-3 adaptor complex mu3A subunit; clathrin adaptor complex AP3, mu-3A subunit; adapter-related protein complex 3 mu-1 subunit; adapter-related protein complex 3 su
• UniProt Protein Name: AP-3 complex subunit mu-1
• Protein Family: AP-3 complex
• UniProt Gene Name: AP3M1
• UniProt Entry Name: AP3M1_HUMAN

NCBI Description

The protein encoded by this gene is the medium subunit of AP-3, which is an adaptor-related protein complex associated with the Golgi region as well as more peripheral intracellular structures. AP-3 facilitates the budding of vesicles from the Golgi membrane and may be directly involved in protein sorting to the endosomal/lysosomal system. AP-3 is a heterotetrameric protein complex composed of two large subunits (delta and beta3), a medium subunit (mu3), and a small subunit (sigma 3). Mutations in one of the large subunits of AP-3 have been associated with the Hermansky-Pudlak syndrome, a genetic disorder characterized by defective lysosome-related organelles. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

AP3M1: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Belongs to the adaptor complexes medium subunit family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: Golgi apparatus; clathrin adaptor complex; cytoplasmic vesicle membrane; lysosomal membrane; lysosome

Molecular Function: protein binding; Rab GTPase binding

Biological Process: protein targeting to lysosome; anterograde synaptic vesicle transport; anterograde axon cargo transport