Western Blot (WB)
(Fig. Western blot analysis of Recombinant AD protein using GAL4 Activation Domain Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)
Rabbit anti-Human GAL4 Activation Domain Polyclonal Antibody
GAL4 Activation Domain Polyclonal Antibody
Reactivity
Human
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
GAL4 Activation Domain; Polyclonal Antibody; GAL4 Activation Domain Polyclonal Antibody; Rabbit Anti-GAL4 Activation Domain Polyclonal Antibody; anti-GAL4 Activation Domain antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody detects endogenous GAL4 Activation Domain protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid; PBS, pH 7.4, Containing 0.02% Sodium Azide As Preservative and 50% Glycerol.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-GAL4 Activation Domain antibody
Western Blot (WB)
Application Notes
WB: 1:10000-1:50000
Immunogen
Recombinant Protein
Preparation and Storage
Stable for one year at -20 degree C from date of shipment.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Western Blot (WB)
(Fig. Western blot analysis of Recombinant AD protein using GAL4 Activation Domain Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)
Western Blot (WB)
(Fig. Western blot analysis of Recombinant AD protein using GAL4 Activation Domain Polyclonal Antibody. Secondary antibody was diluted at 1:20000.)
Related Product Information for anti-GAL4 Activation Domain antibody
The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
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Product Notes
The GAL4 Activation Domain (Catalog #AAA9707850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GAL4 Activation Domain Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GAL4 Activation Domain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:10000-1:50000. Researchers should empirically determine the suitability of the GAL4 Activation Domain for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAL4 Activation Domain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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