Rabbit anti-Human PEK/PERK Polyclonal Antibody | anti-PEK/PERK antibody

PEK/PERK (Ab-981) Antibody

Cat. Num. AAA9408865

• Gene Names: EIF2AK3; PEK; WRS; PERK
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PEK/PERK; Polyclonal Antibody; PEK/PERK (Ab-981) Antibody; E2AK3; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2-alpha kinase 3 precursor; HsPEK; anti-PEK/PERK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total PEK/PERK protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 1116

• Applicable Applications for anti-PEK/PERK antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500~1:3000; Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized non-phosphopeptide derived from human PEK/PERK around the phosphorylation site of threonine 981 (R-H-T(p)-G-Q).
• Target Name: PEK/PERK
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using PEK/PERK (Ab-981) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from MCF-7 cells, using PEK/PERK (Ab-981) antibody.)

Western Blot (WB)

(Western blot analysis of extracts from JK cells (Lane 2), using PEK/PERK (Ab-981) antiobdy. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-PEK/PERK antibody

Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) By similarity.Shi Y., J. Biol. Chem. 274:5723-5730(1999).Sood R., Biochem. J. 346:281-293(2000).Delepine M., Nat. Genet. 25-406-409(2000).

Product Categories/Family for anti-PEK/PERK antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 134304838
• NCBI GeneID: 9451
• NCBI Accession #: NP_004827.4
• NCBI GenBank Nucleotide #: NM_004836.6
• UniProt Accession #: Q9NZJ5; O95846; Q53QY0; Q53SB1; A0AVH1; A0AVH2; B2RCU9
• Molecular Weight: 125,216 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 3 isoform 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2-alpha kinase 3
• NCBI Official Symbol: EIF2AK3
• NCBI Official Synonym Symbols: PEK; WRS; PERK
• NCBI Protein Information: eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Protein Name: Eukaryotic translation initiation factor 2-alpha kinase 3
• UniProt Gene Name: EIF2AK3
• UniProt Synonym Gene Names: PEK; PERK; HsPEK
• UniProt Entry Name: E2AK3_HUMAN

NCBI Description

The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. This protein is thought to modulate mitochondrial function. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Sep 2015]

Uniprot Description

PERK: a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK.

Protein type: Kinase, protein; Translation; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Membrane protein, integral; Other group; PEK family; PEK subfamily

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane

Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; enzyme binding; protein phosphatase binding; eukaryotic translation initiation factor 2alpha kinase activity; ATP binding; protein kinase activity

Biological Process: fat cell differentiation; lactation; positive regulation of protein binding; translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; positive regulation of transcription from RNA polymerase I promoter; cellular response to glucose starvation; regulation of fatty acid metabolic process; angiogenesis; skeletal development; protein homooligomerization; ER overload response; endoplasmic reticulum organization and biogenesis; caspase activation; ossification; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; peptidyl-serine phosphorylation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; insulin-like growth factor receptor signaling pathway; insulin secretion; negative regulation of translation; negative regulation of translation initiation in response to stress; negative regulation of apoptosis

Disease: Epiphyseal Dysplasia, Multiple, With Early-onset Diabetes Mellitus