Endocan Recombinant Protein | ESM1 recombinant protein

Recombinant Endocan

Cat. Num. AAA201092

• Gene Names: ESM1; endocan
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Endocan; Recombinant Endocan; Endothelial cell-specific molecule 1 isoform a; ESM1; ESM1 recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-184aa
• Sequence: ADLWSNNYAV DCPQHCDSSE CKSSPRCKRT VLDDCGCCRV CAAGRGETCY RTVSGMDGMK CGPGLRCQPS NGEDPFGEEF GICKDCPYGT FGMDCRETCN CQSGICDRGT GKCLKFPFFQ YSVTKSSNRF VSLTEHDMAS GDGNIVREEV VKENAAGSPV MRKWLNPRHH HHHH
• Sequence Length: 134

• Applicable Applications for ESM1 recombinant protein: SDS-PAGE
• Species: Human
• Endotoxin: <1.0 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Predicted N-Terminal: Ala
• Preparation and Storage: Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )

Related Product Information for ESM1 recombinant protein

ESM1, also known as endothelial cell-specific molecule 1 isoform a, is a secreted cysteine-rich dermatan sulfate proteoglycan. This protein primarily expressed by endothelial cells within the vascular capillary network in the alveolar walls of the lung and also at lower level in kidney. The expression of it is upregulated by TNF-alpha, IL-beta or lipopolysaccharide and down-regulated by IFN-gamma. It plays roles on angiogenesis, sprouting, and may have potent implications in lung endothelial cell-leukocyte interaction. Also, this protein has dermatan sulfate (DS) that is covalently attached to serine 137. It binds using DS chain to hepatocyte growth factor (HGF) to enhance HGF mitogenic activity. Genetically engineered cells overexpressing ESM1 has been shown to induce tumor formation, suggesting that it may be involved in the pathophysiology of tumor growth in vivo. Recombinant human ESM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for ESM1 recombinant protein

Cancer; Adhesion & Movement

References

Bechard D., et al, (2001) J. Biol. Chem. 276:48341-48349. Scherpereel A., et al. (2003) Cancer Res. 63:6084.

NCBI and Uniprot Product Information

• NCBI GI #: 208022668
• NCBI GeneID: 11082
• NCBI Accession #: NP_001129076.1
• NCBI GenBank Nucleotide #: NP_001129076.1
• UniProt Accession #: Q9NQ30
• Molecular Weight: 19.2kDa (174aa); 18-28kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: endothelial cell-specific molecule 1 isoform b
• NCBI Official Synonym Full Names: endothelial cell specific molecule 1
• NCBI Official Symbol: ESM1
• NCBI Official Synonym Symbols: endocan
• NCBI Protein Information: endothelial cell-specific molecule 1
• UniProt Protein Name: Endothelial cell-specific molecule 1
• Protein Family: Endothelial cell-specific molecule
• UniProt Gene Name: ESM1
• UniProt Synonym Gene Names: ESM-1
• UniProt Entry Name: ESM1_HUMAN

NCBI Description

This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

ESM1: May have potent implications in lung endothelial cell- leukocyte interactions. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: extracellular region

Molecular Function: integrin binding; insulin-like growth factor binding; hepatocyte growth factor receptor binding

Biological Process: positive regulation of cell proliferation; regulation of cell growth; angiogenesis; sprouting angiogenesis