Enoyl CoA Hydratase, Short chain, 1 Active Protein | ECHS1 active protein
Recombinant Human Enoyl CoA Hydratase, Short chain, 1, Mitochondrial, Active
Gene Names
ECHS1; SCEH; ECHS1D
Purity
Greater than 95.0% as determined by SDS-PAGE
Synonyms
Enoyl CoA Hydratase; Short chain; 1; Recombinant Human Enoyl CoA Hydratase; Mitochondrial; Active; Enoyl-CoA Hydratase; Short Chain1; Enoyl Coenzyme A Hydratase; Short Chain; mitochondrial; Short Chain Enoyl-CoA Hydratase; EC 4.2.1.17; SCEH; Short-Chain Enoyl-CoA Hydratase; Enoyl-CoA Hydratase 1; EC 4.2.1; ECHS1D; ECHS1 ; ECHS1 Human; Human Recombinant; ECHS1 active protein
Host
Escherichia Coli
Purity/Purification
Greater than 95.0% as determined by SDS-PAGE
Form/Format
Sterile filtered clear solution
Protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% Glycerol and 100mM NaCl
Protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% Glycerol and 100mM NaCl
Sequence
MGSSHHHHHH SSGLVPRGSH M ASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ
Sequence Length
290
Biological Activity
Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyzes 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25 degree C
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles
Related Product Information for ECHS1 active protein
Description: ECHS1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 284 amino acids (28-290 a.a) and having a molecular mass of 30.6kDa. ECHS1 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: ECHS1 is part of the hydratase/isomerase superfamily. ECHS1 is localized in the mitochondrial matrix and Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that takes part in the second phase of the mitochondrial fatty acid beta-oxidation pathway.
Introduction: ECHS1 is part of the hydratase/isomerase superfamily. ECHS1 is localized in the mitochondrial matrix and Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that takes part in the second phase of the mitochondrial fatty acid beta-oxidation pathway.
Product Categories/Family for ECHS1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,387 Da
NCBI Official Full Name
Enoyl-CoA hydratase, mitochondrial
NCBI Official Synonym Full Names
enoyl-CoA hydratase, short chain 1
NCBI Official Symbol
ECHS1
NCBI Official Synonym Symbols
SCEH; ECHS1D
NCBI Protein Information
enoyl-CoA hydratase, mitochondrial
UniProt Protein Name
Enoyl-CoA hydratase, mitochondrial
Protein Family
UniProt Gene Name
ECHS1
UniProt Synonym Gene Names
SCEH
NCBI Description
The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]
Uniprot Description
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
Research Articles on ECHS1
Similar Products
Product Notes
The ECHS1 echs1 (Catalog #AAA147113) is an Active Protein produced from Escherichia Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH M ASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ. It is sometimes possible for the material contained within the vial of "Enoyl CoA Hydratase, Short chain, 1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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