Dual Specificity Phosphatase 18 Active Protein | DUSP18 active protein

Recombinant Human Dual Specificity Phosphatase 18, Active

Cat. Num. AAA147107

• Gene Names: DUSP18; DSP18; DUSP20; LMWDSP20
• Purity: Greater than 95.0% as determined by SDS-PAGE
• Synonyms: Dual Specificity Phosphatase 18; Recombinant Human Dual Specificity Phosphatase 18; Active; Dual specificity protein phosphatase 18; Low molecular weight dual specificity phosphatase 20; LMW-DSP20; DUSP18; LMWDSP20; VHP; DUSP26; DUSP18 Human; Dual Specificity Phosphatase 18 Human Recombinant; DUSP18 active protein

• Host: Escherichia Coli
• Purity/Purification: Greater than 95.0% as determined by SDS-PAGE
• Form/Format: Sterile filtered clear solution; Protein solution (0.5mg/ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 0.1mM PMSF, 1mM DTT, 40% glycerol and 1mM EDTA
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSH MTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL
• Sequence Length: 188

• Biological Activity: Specific activity is > 300 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37 degree C
• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at-20 degree C for longer periods of time.; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).; Avoid multiple freeze-thaw cycles

Related Product Information for DUSP18 active protein

Description: DUSP18 produced in E Coli is a single, non-glycosylated polypeptide chain containing 212 amino acids (1-188a.a.) and having a molecular mass of 23.6kDa. DUSP18 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction: Dual specificity phosphatase 18 (DUSP18) belongs to the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 has a preferential enzymatic activity for phosphorylated tyrosine residues over threonine residues, in addition DUSP18 dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. Furthermore, DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. DUSP18 is extensively expressed with the highest levels in the liver, brain, ovary and testis.

Product Categories/Family for DUSP18 active protein

Enzymes; Phosphatase; DUSP18 Human; Active

NCBI and Uniprot Product Information

• NCBI GI #: 29840768
• NCBI GeneID: 150290
• UniProt Accession #: Q8NEJ0; B3KPA4
• Molecular Weight: 21,066 Da
• NCBI Official Full Name: Dual specificity protein phosphatase 18
• NCBI Official Synonym Full Names: dual specificity phosphatase 18
• NCBI Official Symbol: DUSP18
• NCBI Official Synonym Symbols: DSP18; DUSP20; LMWDSP20
• NCBI Protein Information: dual specificity protein phosphatase 18
• UniProt Protein Name: Dual specificity protein phosphatase 18
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP18
• UniProt Synonym Gene Names: LMWDSP20; LMW-DSP20

NCBI Description

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP18 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]

Uniprot Description

Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP).