ESAM/Endothelial Cell Adhesion Molecule Recombinant Protein | ESAM recombinant protein

Recombinant Human ESAM/Endothelial Cell Adhesion Molecule Protein (C-His)

Cat. Num. AAA2557301

• Gene Names: ESAM; W117m
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: ESAM/Endothelial Cell Adhesion Molecule; Recombinant Human ESAM/Endothelial Cell Adhesion Molecule Protein (C-His); Endothelial Cell-Selective Adhesion Molecule; ESAM; ESAM recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Sequence: Gln30-Ala247
• Sequence Length: 390

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for ESAM recombinant protein

Protein Construction: Recombinant Human Endothelial Cell Adhesion Molecule is produced by our mammalian expression system and the target gene encoding Gln30-Ala247 is expressed with a 6His tag at the C-terminus.

Background: Endothelial Cell Adhesion Molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein member of the JAM family of immunoglobulin superfamily molecules. The 390 amino acid Human ESAM contains a 216 amino acid extracellular domain (ECD) with a V-type and a C2-type immunoglobulin (Ig) domain. ESAM is specifically expressed at endothelial tight junctions and on activated platelets and performs homophilic adhesion activity. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. In addition, ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. ESAM-deficient mice were described with lowered angiogenic potential, and accordingly, overexpression of ESAM is closely associated with certain tumor growth and metastasis. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes. The ECD of human and mouse ESAM share 69% amino acid identity.

NCBI and Uniprot Product Information

• NCBI GI #: 148664205
• NCBI GeneID: 90952
• NCBI Accession #: NP_620411.2
• NCBI GenBank Nucleotide #: NP_620411.2
• UniProt Accession #: Q96AP7
• Molecular Weight: Molecular Mass: 24.79kDa; Actual Protein Molecular Mass: 38kDa
• NCBI Official Full Name: endothelial cell-selective adhesion molecule
• NCBI Official Synonym Full Names: endothelial cell adhesion molecule
• NCBI Official Symbol: ESAM
• NCBI Official Synonym Symbols: W117m
• NCBI Protein Information: endothelial cell-selective adhesion molecule
• UniProt Protein Name: Endothelial cell-selective adhesion molecule
• Protein Family: Endothelial cell-selective adhesion molecule
• UniProt Gene Name: ESAM

Uniprot Description

Can mediate aggregation most likely through a homophilic molecular interaction.