Syncytin-1 (ERVW-1) Recombinant Protein | ERVW-1 recombinant protein

Recombinant Human Syncytin-1 (ERVW-1), partial

Cat. Num. AAA9423789

• Gene Names: ERVW-1; ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Syncytin-1 (ERVW-1); Recombinant Human Syncytin-1 (ERVW-1); partial; Endogenous retrovirus group W member 1; Env-W; Envelope polyprotein gPr73; Enverin; HERV-7q Envelope protein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; Syncytin; Cleaved into the following 2 chains: Surface protein; Short name: SU; Alternative name(s): gp50 Transmembrane protein; Short name: TM; Alternative name(s): gp24; ERVW-1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Tris-based buffer, 50% glycerol
• Sequence Positions: Extracellular Domain, 21-443aa
• Sequence: APPPCRCMTSSSPYQEFLWRMQRPGNIDAPSYRSLSKGTPTFTAHTHMPRNCYHSATLCMHANTHYWTGKMINPSCPGGLGVTVCWTYFTQTGMSDGGGVQDQAREKHVKEVISQLTRVHGTSSPYKGLDLSKLHETLRTHTRLVSLFNTTLTGLHEVSAQNPTNCWICLPLNFRPYVSIPVPEQWNNFSTEINTTSVLVGPLVSNLEITHTSNLTCVKFSNTTYTTNSQCIRWVTPPTQIVCLPSGIFFVCGTSAYRCLNGSSESMCFLSFLVPPMTIYTEQDLYSYVISKPRNKRVPILPFVIGAGVLGALGTGIGGITTSTQFYYKLSQELNGDMERVADSLVTLQDQLNSLAAVVLQNRRALDLLTAERGGTCLFLGEECCYYVNQSGIVTEKVKEIRDRIQRRAEELRNTGPWGLLSQ

• Immunogen Description: Expression Region:21-443aaSequence Info:Extracellular Domain
• Tag Info: N-terminal 6xHis-SUMO-tagged
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20°C,-80°C. The shelf life of lyophilized form is 12 months at -20°C,-80°C.Notes:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Test Data

Related Product Information for ERVW-1 recombinant protein

This endogenous retroviral envelope protein has retained its original fusogenic properties and participates in trophoblast fusion and the formation of a syncytium during placenta morphogenesis. May induce fusion through binding of SLC1A4 and SLC1A5 (PubMed:10708449, PubMed:12050356, PubMed:23492904).Endogenous envelope proteins may have kept, lost or modified their original function during evolution. Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. The surface protein (SU) mediates receptor recognition, while the transmembrane protein (TM) acts as a class I viral fusion protein. The protein may have at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of membranes.

NCBI and Uniprot Product Information

• NCBI GI #: 195963433
• NCBI GeneID: 30816
• NCBI Accession #: NP_001124397.1
• NCBI GenBank Nucleotide #: NM_001130925.1
• UniProt Accession #: Q9UQF0; O95244; O95245; Q8NHY7; Q9NRZ2; Q9NZG3; B2RPD4
• Molecular Weight: 63 kDa
• NCBI Official Full Name: syncytin-1
• NCBI Official Synonym Full Names: endogenous retrovirus group W member 1, envelope
• NCBI Official Symbol: ERVW-1
• NCBI Official Synonym Symbols: ENV; ENVW; HERVW; ERVWE1; HERV7Q; HERV-7q; HERVWENV; HERV-W-ENV
• NCBI Protein Information: syncytin-1
• UniProt Protein Name: Syncytin-1
• Protein Family: Syncytin
• UniProt Gene Name: ERVW-1
• UniProt Synonym Gene Names: ERVWE1; SU; TM

NCBI Description

Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The gene's protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta. The protein has the characteristics of a typical retroviral envelope protein, including a furin cleavage site that separates the surface (SU) and transmembrane (TM) proteins which form a heterodimer. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

ERVWE1: Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has retained its original fusogenic properties and participates in trophoblast fusion during placenta morphogenesis. Belongs to the gamma type-C retroviral envelope protein family. HERV class-I W env subfamily.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q21.2

Biological Process: anatomical structure morphogenesis; plasma membrane fusion; syncytium formation; syncytium formation by plasma membrane fusion