ERP27 recombinant protein

Recombinant Human ERP27 Protein

Cat. Num. AAA8248991

• Gene Names: ERP27; PDIA8; C12orf46
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Synonyms: ERP27; Recombinant Human ERP27 Protein; C12orf46; Endoplasmic reticulum resident protein 27; ER protein 27; ERp27; ERP27 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: EVEKSSDGPG AAQEPTWLTD VPAAMEFIAA TEVAVIGFFQ DLEIPAVPIL HSMVQKFPGV SFGISTDSEV LTHYNITGNT ICLFRLVDNE QLNLEDEDIE SIDATKLSRF IEINSLHMVT EYNPVTVIGL FNSVIQIHLL LIMNKASPEY EENMHRYQKA AKLFQGKILF ILVDSGMKEN GKVISFFKLK ESQLPALAIY QTLDDEWDTL PTAEVSVEHV QNFCDGFLSG KLLKENRESE GKTPKVELVD HHHHHH
• Sequence Length: 172

• Applicable Applications for ERP27 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ERP27 recombinant protein

Recombinant Human ERP27 Protein is produced by our Mammalian expression system and the target gene encoding Glu26-Leu273 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 663854316
• NCBI GeneID: 121506
• NCBI Accession #: NP_001287713.1
• NCBI GenBank Nucleotide #: NM_001300784.1
• UniProt Accession #: Q96DN0
• Molecular Weight: 30,480 Da
• NCBI Official Full Name: endoplasmic reticulum resident protein 27 isoform 2
• NCBI Official Synonym Full Names: endoplasmic reticulum protein 27
• NCBI Official Symbol: ERP27
• NCBI Official Synonym Symbols: PDIA8; C12orf46
• NCBI Protein Information: endoplasmic reticulum resident protein 27
• UniProt Protein Name: Endoplasmic reticulum resident protein 27
• Protein Family: Endoplasmic reticulum resident protein
• UniProt Gene Name: ERP27
• UniProt Synonym Gene Names: C12orf46; ER protein 27; ERp27
• UniProt Entry Name: ERP27_HUMAN

NCBI Description

This gene encodes a noncatalytic member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. The canonical protein has an N-terminal signal sequence, two thioredoxin (TRX)-like domains and a C-terminal ER-retention sequence. Alternative splicing results in multiple transcript variants encoding distinct isoforms; some of which lack domains present in the canonical protein. [provided by RefSeq, Dec 2016]

Uniprot Description

ERP27: Belongs to the protein disulfide isomerase family. Interacts with PDIA3. Binds somatostatin-14 via hydrophobic interactions

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: endoplasmic reticulum

Molecular Function: protein binding; protein disulfide isomerase activity

Biological Process: protein folding