Porcine Endoplasmic Reticulum To Nucleus Signalling 1 ELISA Kit | ERN1 elisa kit

Porcine Endoplasmic Reticulum To Nucleus Signalling 1 ELISA Kit

Cat. Num. AAA745648

• Gene Names: ERN1; IRE1; IRE1P; IRE1a; hIRE1p
• Reactivity: Porcine
• Synonyms: Endoplasmic Reticulum To Nucleus Signalling 1; Porcine Endoplasmic Reticulum To Nucleus Signalling 1 ELISA Kit; ERN1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This ERN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ERN1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERN1 elisa kit

Principle of the assay: ERN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ERN1 antibody and an ERN1-HRP conjugate. The assay sample and buffer are incubated together with ERN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ERN1 concentration since ERN1 from samples and ERN1-HRP conjugate compete for the anti-ERN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ERN1 from the sample, fewer sites are left to bind ERN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERN1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ERN1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 62089324
• NCBI GeneID: 2081
• Molecular Weight: 6,649 Da
• NCBI Official Full Name: endoplasmic reticulum to nucleus signalling 1 isoform 1 variant, partial
• NCBI Official Synonym Full Names: endoplasmic reticulum to nucleus signaling 1
• NCBI Official Symbol: ERN1
• NCBI Official Synonym Symbols: IRE1; IRE1P; IRE1a; hIRE1p
• NCBI Protein Information: serine/threonine-protein kinase/endoribonuclease IRE1; ire1-alpha; inositol-requiring 1; ER to nucleus signalling 1; inositol-requiring enzyme 1; inositol-requiring protein 1; endoplasmic reticulum-to-nucleus signaling 1
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE1
• UniProt Gene Name: ERN1
• UniProt Synonym Gene Names: IRE1; hIRE1p; IRE1a
• UniProt Entry Name: ERN1_HUMAN

NCBI Description

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Uniprot Description

IRE1: a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1.

Protein type: Endoplasmic reticulum; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Ribonuclease; Protein kinase, Other; Membrane protein, integral; Chaperone; Apoptosis; Other group; IRE family

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cytoplasm; nuclear inner membrane; integral to endoplasmic reticulum membrane

Molecular Function: endoribonuclease activity; protein serine/threonine kinase activity; identical protein binding; protein binding; protein homodimerization activity; enzyme binding; magnesium ion binding; ADP binding; ATP binding

Biological Process: transcription, DNA-dependent; unfolded protein response; protein amino acid autophosphorylation; RNA splicing; protein amino acid phosphorylation; positive regulation of RNA splicing; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; endothelial cell proliferation; UFP-specific transcription factor mRNA processing during unfolded protein response; mRNA catabolic process; cell cycle arrest; regulation of macroautophagy; mRNA cleavage