Western Blot (WB)
(ANAPC4 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ANAPC4 antibody detected the ANAPC4 protein (arrow).)
Rabbit anti-Human ANAPC4 Polyclonal Antibody | anti-ANAPC4 antibody
ANAPC4 Antibody (Center)
Western Blot (WB)
(ANAPC4 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ANAPC4 antibody detected the ANAPC4 protein (arrow).)
Western Blot (WB)
(ANAPC4 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ANAPC4 antibody detected the ANAPC4 protein (arrow).)
complex (APC), or the cyclosome, promotes metaphase-anaphase
transition by ubiquitinating its specific substrates such as
mitotic cyclins and anaphase inhibitor, which are subsequently
degraded by the 26S proteasome. Biochemical studies have shown that
the vertebrate APC contains eight subunits. The composition of the
APC is highly conserved in organisms from yeast to humans. The
exact function of this gene product is not known. [provided by
RefSeq].
Jin, L., et al. Cell 133(4):653-665(2008)
Nature 447(7145):661-678(2007)
Dube, P., et al. Mol. Cell 20(6):867-879(2005)
Listovsky, T., et al. EMBO J. 23(7):1619-1626(2004)
NCBI and Uniprot Product Information
NCBI Description
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Uniprot Description
APC4: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the APC4 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 4p15.2
Cellular Component: nucleoplasm; anaphase-promoting complex; nucleus; cytosol
Molecular Function: protein binding; ubiquitin-protein ligase activity; protein phosphatase binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle
Research Articles on ANAPC4
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Product Notes
The ANAPC4 anapc4 (Catalog #AAA9206929) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 638-664. The ANAPC4 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANAPC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the ANAPC4 anapc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANAPC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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