Rat Estrogen Receptor Alpha ELISA Kit | ERalpha elisa kit

Rat Estrogen Receptor Alpha ELISA Kit

Cat. Num. AAA726536

• Reactivity: Rat
• Synonyms: Estrogen Receptor Alpha; Rat Estrogen Receptor Alpha ELISA Kit; ERalpha elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/ml
• Intended Uses: This ERalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ERalpha. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ERalpha elisa kit

Principle of the Assay: ERalpha ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ERalpha. Standards or samples are then added to the microtiter plate wells and ERalpha if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ERalpha present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ERalpha are added to each well to "sandwich" the ERalpha immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ERalpha and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ERalpha concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ERalpha elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 5821726
• Molecular Weight: 35,547 Da
• NCBI Official Full Name: estrogen receptor alpha, partial
• UniProt Protein Name: Estrogen receptor
• UniProt Gene Name: ESR1
• UniProt Synonym Gene Names: ESR; NR3A1; ER
• UniProt Entry Name: ESR1_HUMAN

Uniprot Description

ER-alpha: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; integral to membrane; plasma membrane; nucleus

Molecular Function: identical protein binding; estrogen receptor activity; zinc ion binding; nitric-oxide synthase regulator activity; beta-catenin binding; estrogen response element binding; transcription factor binding; steroid binding; protein binding; enzyme binding; steroid hormone receptor activity; chromatin binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; estrogen receptor signaling pathway; uterus development; signal transduction; response to estradiol stimulus; regulation of apoptosis; positive regulation of fibroblast proliferation; elevation of cytosolic calcium ion concentration; regulation of transcription, DNA-dependent; epithelial cell development; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; negative regulation of transcription factor activity; positive regulation of nitric-oxide synthase activity; antral ovarian follicle growth; androgen metabolic process; male gonad development; vagina development; positive regulation of retinoic acid receptor signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; chromatin remodeling; response to estrogen stimulus; gene expression; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of transcription factor activity; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter

Disease: Breast Cancer; Migraine With Or Without Aura, Susceptibility To, 1; Myocardial Infarction, Susceptibility To; Estrogen Resistance