Typical Testing Data/Standard Curve (for reference only)
Human Succinate Dehydrogenase Complex Subunit A (SDHA) ELISA Kit | SDHA elisa kit
Human Succinate Dehydrogenase Complex Subunit A (SDHA) ELISA Kit
Gene Names
SDHA; FP; PGL5; SDH1; SDH2; SDHF; CMD1GG
Reactivity
Human
Synonyms
Succinate Dehydrogenase Complex Subunit A (SDHA); Human Succinate Dehydrogenase Complex Subunit A (SDHA) ELISA Kit; SDH2; SDHF; FP; Flavoprotein; Flavoprotein subunit of complex II; Succinate dehydrogenase [ubiquinone] flavoprotein subunit; mitochondrial; SDHA elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SDHA. No significant cross-reactivity or interference between SDHA and analogues was observed.
Sequence Length
616
Samples
Tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
78.125-5000pg/mL
Sensitivity
<33.2pg/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SDHA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SDHA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SDHA elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SDHA in human tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDHA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SDHA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SDHA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SDHA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SDHA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SDHA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SDHA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,650 Da
NCBI Official Full Name
succinate dehydrogenase
NCBI Official Synonym Full Names
succinate dehydrogenase complex flavoprotein subunit A
NCBI Official Symbol
SDHA
NCBI Official Synonym Symbols
FP; PGL5; SDH1; SDH2; SDHF; CMD1GG
NCBI Protein Information
succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
UniProt Protein Name
Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
Protein Family
UniProt Gene Name
SDHA
UniProt Synonym Gene Names
SDH2; SDHF; Fp
NCBI Description
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
Uniprot Description
Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:24781757). Can act as a tumor suppressor (PubMed:20484225).
Research Articles on SDHA
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Product Notes
The Human SDHA sdha (Catalog #AAA457157) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457157 ELISA Kit recognizes Human SDHA. It is sometimes possible for the material contained within the vial of "Succinate Dehydrogenase Complex Subunit A (SDHA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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