EphB1 / EPHT2 recombinant protein

Recombinant Mouse EphB1 / EPHT2 Protein (His tag)

Cat. Num. AAA2546960

• Gene Names: Ephb1; Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: EphB1 / EPHT2; Recombinant Mouse EphB1 / EPHT2 Protein (His tag); 9330129L11; AW488255; C130099E04Rik; Cek6; Elk; Elkh; Hek6; Net; EphB1 / EPHT2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 943

• Application Notes: The secreted recombinant mouse EPHB1 consists of 533 amino acids and has a predicted molecular mass of 59.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EPHB1 is approximately 64 kDa due to glycosylation.
• Predicted N Terminal: Met 18
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for EphB1 / EPHT2 recombinant protein

Background: Ephrin type -B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Description: A DNA sequence encoding the mouse EPHB1 (Q8CBF3-1) extracellular domain (Met 1-Leu 539) was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 269973848
• NCBI GeneID: 270190
• NCBI Accession #: NP_001161768.1
• NCBI GenBank Nucleotide #: NM_001168296.1
• UniProt Accession #: Q8CBF3; Q3UY27; Q6PG23; Q8CBE2; B1B1C2
• Molecular Weight: 105,294 Da
• NCBI Official Full Name: ephrin type-B receptor 1 isoform 2
• NCBI Official Synonym Full Names: Eph receptor B1
• NCBI Official Symbol: Ephb1
• NCBI Official Synonym Symbols: Elk; Net; Cek6; Elkh; Hek6; AW488255; 9330129L11; C130099E04Rik
• NCBI Protein Information: ephrin type-B receptor 1
• UniProt Protein Name: Ephrin type-B receptor 1
• UniProt Gene Name: Ephb1

Uniprot Description

EphB1: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-B family: ephrin-B1, -B2 and -B3. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB1 interacts with GRB2, GRB10 and NCK through their respective SH2 domains. Four alternatively spliced isoforms are known.

Protein type: EC 2.7.10.1; Eph family; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 9|9 F1

Cellular Component: axon; cytoplasm; early endosome membrane; filopodium tip; integral to plasma membrane; lipid raft; membrane

Molecular Function: axon guidance receptor activity; protein binding; protein complex binding; protein-tyrosine kinase activity; transmembrane-ephrin receptor activity

Biological Process: angiogenesis; axon guidance; camera-type eye morphogenesis; cell-substrate adhesion; central nervous system projection neuron axonogenesis; cranial nerve development; detection of temperature stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; establishment of cell polarity; formation of immunological synapse; neurogenesis; optic nerve morphogenesis; positive regulation of synaptogenesis; protein amino acid autophosphorylation; regulation of JNK cascade; retinal ganglion cell axon guidance