ENPP-5 ELISA Pair Set | ENPP5 elisa pair set

ENPP-5 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118377

• Synonyms: ENPP-5; ENPP-5 Matched ELISA Antibody Pair Set; Human; Human ENPP5 ELISA Pair Set; ectonucleotide pyrophosphatase/phosphodiesterase 5 (putative); NPP-5 Matched ELISA Antibody Pair Set; ENPP5 elisa pair set

• Specificity: Quantitative determination of Human ENPP-5

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 46.88-3000pg/mL
• Sensitivity: 46.88pg/ml
• Materials Provided: 1. Capture Antibody 0.5 mg/mL of rabbit anti-Human ENPP5 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human ENPP5 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 75 ng of recombinant Human ENPP5. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for ENPP5 elisa pair set

ENPP5 is a member of the nucleotide pyrophosphatase/phosphodiesterase family (NPP). It is a family comprised of dimeric enzymes that catalyze the hydrolysis of phosphate diester bonds. There are seven isoforms in NPP family, some of which prefer nucleotide substrates, some of which prefer phospholipid substrates, and others of which prefer substrates that have not yet been determined. NPP also belongs to the alkaline phosphatase (AP) superfamily of enzymes and they are located in the cell membrane and hydrolyze extracellular phosphate diesters to affect a wide variety of biological processes. ENPP5 belongs to a group of nucleotidemetabolizing ectoenzymes, which regulate the availability of extracellular nucleotides. ENPP5 may play a role in neuronal cell communication. However, it lacks nucleotide pyrophosphatase and lysopholipase D activity. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat. ENPP5 functions in phospholipid metabolism.

This ENPP-5 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human ENPP-5. It contains Human ENPP-5 capture antibody, Human ENPP-5 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human ENPP-5 protein.

Product Categories/Family for ENPP5 elisa pair set

Phosphatases and Regulators

References

Strausberg RL, et al. (2002) Generation and initial analysis of more than 15, 000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.Gerhard DS, et al. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14(10B):2121-7.Lamesch P, et al. (2007) hORFeome v3.1: a resource of human open reading frames representing over 10, 000 human genes. Genomics. 89(3):307-15.