Typical Testing Data/Standard Curve (for reference only)
Canine Trefoil Factor 3, Intestinal ELISA Kit | TFF3 elisa kit
Canine Trefoil Factor 3, Intestinal ELISA Kit
Gene Names
Tff3; ITF
Reactivity
Canine
Synonyms
Trefoil Factor 3; Intestinal; Canine Trefoil Factor 3; Intestinal ELISA Kit; TFF3 elisa kit
Reactivity
Canine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Intended Uses
This TFF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TFF3. This ELISA kit for research use only, not for therapeutic applications
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TFF3 elisa kit
Principle of the Assay: TFF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TFF3 antibody and an TFF3-HRP conjugate. The assay sample and buffer are incubated together with TFF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TFF3 concentration since TFF3 from samples and TFF3-HRP conjugate compete for the anti-TFF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TFF3 from the sample, fewer sites are left to bind TFF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TFF3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for TFF3 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,944 Da
NCBI Official Full Name
trefoil factor 3
NCBI Official Synonym Full Names
trefoil factor 3, intestinal
NCBI Official Symbol
Tff3
NCBI Official Synonym Symbols
ITF
NCBI Protein Information
trefoil factor 3; polypeptide P1.B; intestinal trefoil factor
UniProt Protein Name
Trefoil factor 3
Protein Family
UniProt Gene Name
Tff3
UniProt Synonym Gene Names
Itf; rITF; rP1.B
UniProt Entry Name
TFF3_RAT
NCBI Description
plays a role in epithelial wound healing; involved in maintenance of the intestinal mucosa [RGD, Feb 2006]
Uniprot Description
Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) ().
Research Articles on TFF3
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Product Notes
The Canine TFF3 tff3 (Catalog #AAA734223) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA734223 ELISA Kit recognizes Canine TFF3. It is sometimes possible for the material contained within the vial of "Trefoil Factor 3, Intestinal, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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