PVRL1/NECTIN1 ELISA Pair Set | NECTIN1 elisa pair set

PVRL1/NECTIN1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118386

• Synonyms: PVRL1/NECTIN1; PVRL1/NECTIN1 Matched ELISA Antibody Pair Set; Human; Human CD111/Nectin-1/PVRL1 ELISA Pair Set; poliovirus receptor-related 1 (herpesvirus entry mediator C); CD111 Matched ELISA Antibody Pair Set; CLPED1 Matched ELISA Antibody Pair Set; ED4 Matched ELISA Antibody Pair Set; HIgR Matched ELISA Antibody Pair Set; HV1S Matched ELISA Antibody Pair Set; HVEC Matched ELISA Antibody Pair Set; nectin-1 Matched ELISA Antibody Pair Set; OFC7 Matched ELISA Antibody Pair Set; PRR Matched ELISA Antibody Pair Set; PRR1 Matched ELISA Antibody Pair Set; PVRR Matched ELISA Antibody Pair Set; PVRR1 Matched ELISA Antibody Pair Set; SK-12 Matched ELISA Antibody Pair Set; NECTIN1 elisa pair set

• Specificity: Quantitative determination of Human PVRL1/NECTIN1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 5000pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human CD111/Nectin-1/PVRL1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human CD111/Nectin-1/PVRL1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 140 ng of recombinant Human CD111/Nectin-1/PVRL1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 78.125 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for NECTIN1 elisa pair set

Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

This PVRL1/NECTIN1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human PVRL1/NECTIN1. It contains Human PVRL1/NECTIN1 capture antibody, Human PVRL1/NECTIN1 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human PVRL1/NECTIN1 protein.

References

Yoon M, et al. (2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8.Mata M, et al. (2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80.Haarr L, et al. (2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.