LEC/CCL16 ELISA Pair Set | CCL16 elisa pair set

LEC/CCL16 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118261

• Synonyms: LEC/CCL16; LEC/CCL16 Matched ELISA Antibody Pair Set; Human; Human CCL16/HCC-4/NCC4 ELISA Pair Set; chemokine (C-C motif) ligand 16; CCL16 Matched ELISA Antibody Pair Set; CKb12 Matched ELISA Antibody Pair Set; HCC-4 Matched ELISA Antibody Pair Set; ILINCK Matched ELISA Antibody Pair Set; LCC-1 Matched ELISA Antibody Pair Set; LEC Matched ELISA Antibody Pair Set; LMC Matched ELISA Antibody Pair Set; Mtn-1 Matched ELISA Antibody Pair Set; NCC-4 Matched ELISA Antibody Pair Set; NCC4 Matched ELISA Antibody Pair Set; SCYA16 Matched ELISA Antibody Pair Set; SCYL4 Matched ELISA Antibody Pair Set; CCL16 elisa pair set

• Specificity: Quantitative determination of Human LEC/CCL16

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 2.34-150pg/mL
• Sensitivity: 2.34pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human CCL16/HCC-4/NCC4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human CCL16/HCC-4/NCC4 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use.3. Standard Each vial contains 10 (KW10JL1501-1) ng of recombinant Human CCL16/HCC-4/NCC4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 150 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for CCL16 elisa pair set

CCL16, a chemokine poorly characterized at the functional level. Human CCL16 is a member of the CC family, and its gene maps to human chromosome 17q. In the mouse, only a pseudogene has been identified to date. CCL16 is a functional ligand for CCR1, CCR2, CCR5, and CCR8. Recombinant CCL16 demonstrated chemotactic activity on human monocytes and lymphocytes. Based on the ability of human chemokines to exert activity on and bind to murine receptors, the TSA mouse adenocarcinoma cell line was transfected with human CCL16 cDNA and, in comparison with other cytokines, was shown to be the faster inducer of systemic immune response due to massive, prompt infiltration of leukocytes.

This LEC/CCL16 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human LEC/CCL16. It contains Human LEC/CCL16 capture antibody, Human LEC/CCL16 detector antibody (HRP) and a highly purified E Coli-expressed recombinant Human LEC/CCL16 protein.

References

Guiducci C, Di Carlo E, Parenza M, et al. Intralesional injection of adenovirus encoding CC chemokine ligand 16 inhibits mammary tumor growth and prevents metastatic-induced death after surgical removal of the treated primary tumor[J]. The Journal of Immunology, 2004, 172(7): 4026-4036.