Testing Data
IL1R2 elisa pair set
IL1R2 Matched ELISA Antibody Pair Set, Human
Synonyms
IL1R2; IL1R2 Matched ELISA Antibody Pair Set; Human; Human IL1R2/IL1RB/CD121b ELISA Pair Set; interleukin 1 receptor; type II; CD121b Matched ELISA Antibody Pair Set; CDw121b Matched ELISA Antibody Pair Set; IL-1R-2 Matched ELISA Antibody Pair Set; IL-1RT-2 Matched ELISA Antibody Pair Set; IL-1RT2 Matched ELISA Antibody Pair Set; IL1R2c Matched ELISA Antibody Pair Set; IL1RB Matched ELISA Antibody Pair Set; IL1R2 elisa pair set
Specificity
Quantitative determination of Human IL1R2
Assay Type
Solid Phase Sandwich ELISA
Detection Range
9.38-600pg/mL
Sensitivity
9.375pg/ml
Materials Provided
1. Capture Antibody 0.5 mg/mL of rabbit anti-IL1R2 monoclonal antibody. Dilute to a working concentration of 2.0ug/mL in CBS before coating
2. Detection Antibody 0.5 mg/mL mouse anti-IL1R2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 24 ng of recombinant IL1R2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.
2. Detection Antibody 0.5 mg/mL mouse anti-IL1R2 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0ug/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 24 ng of recombinant IL1R2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.
Preparation and Storage
Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20 degree C to -80 degree C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for IL1R2 elisa pair set
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.
This IL1R2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL1R2. It contains Human IL1R2 capture antibody, Human IL1R2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL1R2 protein.
This IL1R2 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human IL1R2. It contains Human IL1R2 capture antibody, Human IL1R2 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human IL1R2 protein.
Product Categories/Family for IL1R2 elisa pair set
References
Cannon JG, et al. (1997) Interleukin-1 beta, interleukin-1 receptor antagonist, and soluble interleukin-1 receptor type II secretion in chronic fatigue syndrome. J Clin Immunol. 17 (3): 253-61.Liu C, et al. (1996) Cloning and characterization of an alternatively processed human type II interleukin-1 receptor mRNA. J Biol Chem. 271 (34): 20965-72.Van der Poll T, et al. (1997) Antiinflammatory cytokine responses during clinical sepsis and experimental endotoxemia: sequential measurements of plasma soluble interleukin (IL)-1 receptor type II, IL-10, and IL-13. J Infect Dis. 175 (1): 118-22.
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Product Notes
The IL1R2 (Catalog #AAA8118186) is an ELISA Pair Set and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "IL1R2, ELISA Pair Set" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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