CD160 recombinant protein

Recombinant Human CD160 Protein (His tag)

Cat. Num. AAA2545547

• Gene Names: CD160; NK1; BY55; NK28
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: CD160; Recombinant Human CD160 Protein (His tag); RP11-373C9.1; BY55; FLJ46513; NK1; NK28; CD160 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 181

• Application Notes: The recombinant human CD160 consists of 143 amino acids and has a predicted molecular mass of 16.4 kDa. In SDS-PAGE under reducing conditions, rh CD160 migrates as an approximately 25 kDa band due to glycosylation.
• Predicted N Terminal: Ile 27
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD160 recombinant protein

Background: CD160 antigen, also known as Natural killer cell receptor BY55 and CD160, is a cell membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD160 is a GPI-anchored lymphocyte surface receptor in which expression is mostly restricted to the highly cytotoxic CD56 (dim)CD16 (+) peripheral blood NK subset. CD160 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD160 is expressed in spleen, peripheral blood, and small intestine. Expression of CD160 is restricted to functional NK and T cytotoxic lymphocytes. CD160 acts as a co-activator receptor for CD3-induced proliferation of CD4+ CD160+ T cells isolated from inflammatory skin lesions. Unique CD4+ CD160+ lymphocyte subset may play a role in the pathogenesis of skin inflammation. Activated NK lymphocytes release a soluble form of CD160 that functionally impairs the MHC-I-specific cytotoxic CD8 (+) T lymphocyte responsiveness.

Description: A DNA sequence encoding the human CD160 (NP_008984.1) (Met 1-Leu 158) without the propeptide was expressed, with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 5901910
• NCBI GeneID: 11126
• NCBI Accession #: NP_008984.1
• NCBI GenBank Nucleotide #: NM_007053.3
• UniProt Accession #: O95971
• Molecular Weight: 19,810 Da
• NCBI Official Full Name: CD160 antigen
• NCBI Official Synonym Full Names: CD160 molecule
• NCBI Official Symbol: CD160
• NCBI Official Synonym Symbols: NK1; BY55; NK28
• NCBI Protein Information: CD160 antigen
• UniProt Protein Name: CD160 antigen
• Protein Family: CD160 antigen
• UniProt Gene Name: CD160
• UniProt Synonym Gene Names: BY55

NCBI Description

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

Receptor showing broad specificity for both classical and non-classical MHC class I molecules.