Mouse anti-Human CD160 Monoclonal Antibody | anti-CD160 antibody

Anti-CD160 Antibody, Mouse Monoclonal

Cat. Num. AAA8106258

• Gene Names: CD160; NK1; BY55; NK28
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD160; Monoclonal Antibody; Anti-CD160 Antibody; Mouse Monoclonal; CD160/NK1/BY55 Antibody; Mouse MAb; CD160 molecule; Anti-BY55 Antibody; Anti-NK1 Antibody; Anti-NK28 Antibody; anti-CD160 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 09
• Specificity: Human CD160/NK1/BY55
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD160 antibody: Flow Cytometry (FC/FACS/FCM)
• Application Notes: FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Human CD160/NK1/BY55 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD160/NK1/BY55 (rh CD160/NK1/BY55). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD160 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD160, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

Related Product Information for anti-CD160 antibody

CD16 antigen, also known as Natural killer cell receptor BY55 and CD16, is a cell membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD16 is a GPI-anchored lymphocyte surface receptor in which expression is mostly restricted to the highly cytotoxic CD56(dim)CD16(+) peripheral blood NK subset. CD16 is a receptor showing broad specificity for both classical and non-classical MHC class I molecules. CD16 is expressed in spleen, peripheral blood, and small intestine. Expression of CD16 is restricted to functional NK and T cytotoxic lymphocytes. CD16 acts as a co-activator receptor for CD3-induced proliferation of CD4+ CD16+ T cells isolated from inflammatory skin lesions. Unique CD4+ CD16+ lymphocyte subset may play a role in the pathogenesis of skin inflammation. Activated NK lymphocytes release a soluble form of CD16 that functionally impairs the MHC-I-specific cytotoxic CD8(+) T lymphocyte responsiveness.

Product Categories/Family for anti-CD160 antibody

Cancer Drug Targets

References

Barakonyi A. et al., 2004, J Immunol.173 (9): 5349-54. Rabot M. et al., 2006, Transpl Immunol. 17 (1): 36-8. Abecassis S. et al., 2007, J Invest Dermatol. 127?(5): 1161-6. Giustiniani J.?et al., 2007, J Immunol. 178 (3): 1293-300.

NCBI and Uniprot Product Information

• NCBI GI #: 5901910
• NCBI GeneID: 11126
• NCBI Accession #: NP_008984.1
• NCBI GenBank Nucleotide #: NM_007053.2
• UniProt Accession #: O95971
• Molecular Weight: 19,810 Da
• NCBI Official Full Name: CD160 antigen
• NCBI Official Synonym Full Names: CD160 molecule
• NCBI Official Symbol: CD160
• NCBI Official Synonym Symbols: NK1; BY55; NK28
• NCBI Protein Information: CD160 antigen; CD160-delta Ig; CD160 transmembrane isoform; natural killer cell receptor BY55; natural killer cell receptor, immunoglobulin superfamily member
• UniProt Protein Name: CD160 antigen
• Protein Family: CD160 antigen
• UniProt Gene Name: CD160
• UniProt Synonym Gene Names: BY55
• UniProt Entry Name: BY55_HUMAN

NCBI Description

CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor showing broad specificity for both classical and non-classical MHC class I molecules. Ref.3

Subunit structure: Homomultimer; disulfide-linked.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor.

Tissue specificity: Expressed in spleen, peripheral blood, and small intestine. Expression is restricted to functional NK and T cytotoxic lymphocytes.

Sequence similarities: Contains 1 Ig-like V-type (immunoglobulin-like) domain.