Thyroxine ELISA Kit
Thyroxine ELISA Kit
Applications
ELISA
Synonyms
Thyroxine; Thyroxine ELISA Kit; Free T4 ELISA Kit; Thyroxine elisa kit
Applicable Applications for Thyroxine elisa kit
ELISA (EIA)
Application Notes
The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient's serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4.
Biological Significance
The thyroid hormone, thyroxine (T4) is produced by the thyroid gland. An important component in the synthesis is iodine. The major form of thyroid hormone in the blood is thyroxine (T4). Thyroxine is converted to the active T3 (three to four times more potent than T4) within cells by deiodinases (5'- iodinase). Thyroxine-binding globulin (TGB) is the major carrier protein for circulating thyroid hormone. Only a very
Biohazard Information
All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV, and HbsAg. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
Preparation and Storage
Store at 4 degree C.
Related Product Information for Thyroxine elisa kit
ELISA kit for detection of Thyroxine in the research laboratory
Product Categories/Family for Thyroxine elisa kit
Similar Products
Product Notes
The Thyroxine (Catalog #AAA539711) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Thyroxine can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). The CBI fT4 is a solid phase competitive ELISA. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. fT4 in the patient's serum competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound fT4 and T4 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of fT4 in the samples. A standard curve is prepared relating color intensity to the concentration of fT4. Researchers should empirically determine the suitability of the Thyroxine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroxine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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