Mouse anti-Transfected GADD45alpha Monoclonal Antibody | anti-GADD45A antibody

GADD45alpha Antibody

Cat. Num. AAA9215505

• Gene Names: GADD45A; DDIT1; GADD45
• Reactivity: Transfected
• Applications: Western Blot
• Synonyms: GADD45alpha; Monoclonal Antibody; GADD45alpha Antibody; Purified Mouse Monoclonal Antibody (Mab); DDIT 1; DDIT-1; DDIT1; DNA damage inducible transcript 1; DNA damage-inducible transcript 1 protein; GA45A_HUMAN; GADD45; GADD45A; Growth arrest and DNA damage inducible 45 alpha; Growth arrest and DNA damage inducible alpha; Growth arrest and DNA damage-inducible protein GADD45 alpha.; anti-GADD45A antibody

• Host: Mouse
• Reactivity: Transfected
• Clonality: Monoclonal
• Isotype: IgG1
• Form/Format: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Sequence Length: 131

• Applicable Applications for anti-GADD45A antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:1000
• Calculated MW: 22 kDa
• Preparation and Storage: Store at -20°C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of GADD45 alpha in CHO-K1 cell lysate(B)and CHO-K1 transfected by GADD45 alpha His fusion protein(A)cell lysate using GADD45 alpha mouse mAb (1:1000 diluted). Predicted band size:22KDa. Observed band size:22KDa.)

Related Product Information for anti-GADD45A antibody

In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.

References

Papathanasiou M.A.,et al.Mol. Cell. Biol. 11:1009-1016(1991). Hollander M.C.,et al.J. Biol. Chem. 268:24385-24393(1993). Zhang Y.,et al.Nucleic Acids Res. 34:485-495(2006). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 315075323
• NCBI GeneID: 1647
• NCBI Accession #: NP_001186670.1
• NCBI GenBank Nucleotide #: NM_001199741.1
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 2
• NCBI Official Synonym Full Names: growth arrest and DNA damage inducible alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• Protein Family: Growth arrest and DNA damage-inducible protein
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1
• UniProt Entry Name: GA45A_HUMAN

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding

Biological Process: positive regulation of apoptosis; apoptosis; activation of MAPKKK activity; centrosome cycle; DNA damage response, signal transduction; positive regulation of JNK cascade; negative regulation of protein kinase activity; regulation of cyclin-dependent protein kinase activity; DNA repair; G2/M transition of mitotic cell cycle; cell cycle arrest