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Syphilis anti-TP ELISA Kit

Syphilis anti-TP ELISA Kit

Synonyms
Syphilis anti-TP; Syphilis anti-TP ELISA Kit; Syphilis anti-TP elisa kit
Ordering
For Research Use Only!
Assay Type
Qualitative Sandwich
Preparation and Storage
+4C
Related Product Information for Syphilis anti-TP elisa kit
Principle of the Assay: With this kit, the detection of anti-TP antibodies is achieved by antigen "sandwich" enzyme-linked method (ELISA) where polystyrene microwell strips are pre-coated with recombinant Treponema pallidum antigens expressed in E. coli. The sample is incubated in the microwells together with recombinant TP antigens conjugated to horseradish peroxidase (HRP-Conjugate). The pre-coated antigens express the same epitopes as the HRP-Conjugate antigens, but are expressed in different hosts. In case of presence of anti-TP in the sample, during incubation the pre-coated and conjugated antigens will be bound to the two variable domains of the antibody and the specific antigens-antibody immunocomplex is captured on the solid phase. After washing to remove sample and unbound conjugates, Chromogen solutions containing tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antigen-antibody-antigen "sandwich" complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color can be measured and is proportional to the amount of antibody in the sample. Wells containing samples negative for anti-TP remain colorless.

Background/Introduction: This kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of the antibodies to Syphilis in human serum or plasma.
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Product Notes

The Syphilis anti-TP (Catalog #AAA412755) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Syphilis anti-TP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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