Testing Data
QuickTiter MuLV Core Antigen ELISA Kit
QuickTiter MuLV Core Antigen ELISA Kit
Synonyms
QuickTiter MuLV Core Antigen; QuickTiter MuLV Core Antigen ELISA Kit; QuickTiter MuLV Core Antigen elisa kit
Preparation and Storage
Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20 degree C to avoid multiple freeze/thaw cycles. Store all other kit components at 4 degree C until their expiration dates.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for QuickTiter MuLV Core Antigen elisa kit
Principle of the assay: An anti-MuLV p30 monoclonal coating antibody is adsorbed onto a microtiter plate. MuLV core antigen (p30) present in the sample or standard binds to the antibodies adsorbed on the plate; an anti-MuLV p30 polyclonal antibody is added and binds to the antigen captured by the first antibody. Following incubation and wash steps, a HRP-conjugated secondary antibody is added and binds to the anti-MuLV p30 polyclonal. Unbound HRP-conjugated secondary antibody is removed during the wash steps, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of MuLV core antigen present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from recombinant MuLV p30 core antigen and sample concentration is then determined.
Background: Murine leukemia viruses (MuLVs or MLVs) are retroviruses known for their ability to infect and cause cancer in murine hosts and other vertebrates. Moloney murine leukemia virus-based retroviral vectors are widely used for gene transfer to various target cells. Recently discovered in humans, xenotropic murine leukemia virus-related virus (XMRV) is closely related to murine leukemia viruses (e.g. Gag p30 core antigens share 96% identity). Early studies have linked XMRV to prostate cancer and chronic fatigue sydrome; however, new findings have disputed these claims. QuickTiter MuLV Core Antigen ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the MuLV core antigen protein (Gag p30). A monoclonal antibody to MuLV p30 is coated onto strip wells of microtiter plate. The quantity of MuLV core antigen in the sample is determined by comparing its absorbance with that of known recombinant MuLV p30 core antigen standard curve. The kit has a detection sensitivity limit of 300 pg/mL MuLV p30. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and MuLV samples.
Product Categories/Family for QuickTiter MuLV Core Antigen elisa kit
References
1. Stoye, JP, Coffin, JM (1987) J Virol. 61: 2659-69.
2. D'Souza V, Dey A, Habib D, Summers MF (2004) J Mol. Biol. 337: 427-42.
3. D'Souza V, Summers MF (2004) Nature 431: 586-90.
4. Urisman A, Molinaro RJ, Fischer N, et al. (2006) PLoS Pathogens 2: e25.
1. Kirchmeier, M. et al. (2014). Enveloped Virus-Like Particle Expression of Human Cytomegalovirus Glycoprotein B Antigen Induces Antibodies with Potent and Broad Neutralizing Activity. Clin. Vaccine Immunol. 21:174-180.
2. Belanger, K. et al. (2013). Binding of RNA by APOBEC3G Controls Deamination-Independent Restriction of Retroviruses. J. Exp. Biol. 216:2213-2220.
2. D'Souza V, Dey A, Habib D, Summers MF (2004) J Mol. Biol. 337: 427-42.
3. D'Souza V, Summers MF (2004) Nature 431: 586-90.
4. Urisman A, Molinaro RJ, Fischer N, et al. (2006) PLoS Pathogens 2: e25.
1. Kirchmeier, M. et al. (2014). Enveloped Virus-Like Particle Expression of Human Cytomegalovirus Glycoprotein B Antigen Induces Antibodies with Potent and Broad Neutralizing Activity. Clin. Vaccine Immunol. 21:174-180.
2. Belanger, K. et al. (2013). Binding of RNA by APOBEC3G Controls Deamination-Independent Restriction of Retroviruses. J. Exp. Biol. 216:2213-2220.
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Product Notes
The QuickTiter MuLV Core Antigen (Catalog #AAA168641) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "QuickTiter MuLV Core Antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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