Kininogen ELISA Kit | KNG1 elisa kit

Human Kininogen total antigen assay ELISA kit

Cat. Num. AAA136014

• Gene Names: KNG1; BK; BDK; KNG
• Synonyms: Kininogen; Human Kininogen total antigen assay ELISA kit; KNG1 elisa kit

• Sequence Length: 427

• Preparation and Storage: Store at 4 degree C

Testing Data

Related Product Information for KNG1 elisa kit

High molecular weight kininogen (HK, aka Fitzgerald Factor) is the single chain 626 amino acid 120kDa glycoprotein precursor of the vasoactive peptide bradykinin. Kininogen is an important cofactor for the activation of zymogens prekallikrein, Factor XII, and Factor XI in the contact activation or intrinsic coagulation pathway. Additionally kininogen is a major inhibitor of systemic cysteine proteinases such cathepsins, calpain and papain. The sensitive quantitative measurement of total human kininogen antigen in plasma and serum samples is easily performed with this 96 well strip format ELISA kit. The concentration of kininogen in normal human plasma is 65-115 ug/ml with an average concentration of 83 ug/ml. The assay measures human kininogen in the 0.07-70 ng/ml range. Samples giving human kininogen levels above 70 ug/ml should be diluted in blocking buffer before use. A 1:10,000-1:50,000 dilution for plasma is suggested for best results. Human kininogen will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase conjugated polyclonal anti-human kininogen primary antibody binds to the captured protein. Following an additional washing step, TMB substrate is used for color development at 450nm. Color development is proportional to the concentration of kininogen in the samples. A standard calibration curve is prepared using dilutions of purified kininogen and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for KNG1 elisa kit

CLOTTING FACTOR ELISA Kits; Human Kininogen ELISA Kits; Human Kits

NCBI and Uniprot Product Information

• NCBI GI #: 386853
• NCBI GeneID: 3827
• Molecular Weight: 43,822 Da
• NCBI Official Full Name: kininogen
• NCBI Official Synonym Full Names: kininogen 1
• NCBI Official Symbol: KNG1
• NCBI Official Synonym Symbols: BK; BDK; KNG
• NCBI Protein Information: kininogen-1
• UniProt Protein Name: Kininogen-1
• Protein Family: Kininogen
• UniProt Gene Name: KNG1
• UniProt Synonym Gene Names: BDK; KNG; HMWK
• UniProt Entry Name: KNG1_HUMAN

Uniprot Description

KNG1: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin- induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW- kininogen is in contrast to HMW-kininogen not involved in blood clotting. Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency). HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Contractile; Inhibitor; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular region; extracellular space; plasma membrane

Molecular Function: cysteine protease inhibitor activity; protein binding; receptor binding

Biological Process: blood coagulation, intrinsic pathway; elevation of cytosolic calcium ion concentration; negative regulation of blood coagulation; negative regulation of cell adhesion; negative regulation of proteolysis; platelet degranulation

Disease: High Molecular Weight Kininogen Deficiency