Rabbit Periostin C-terminal Polyclonal Antibody | anti-POSTN antibody

Anti-Periostin, C-terminal

Cat. Num. AAA502166

• Gene Names: Postn; PN; PLF; Osf2; peri; OSF-2; AI747096; A630052E07Rik
• Reactivity: Chicken, human, mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: Periostin C-terminal; Polyclonal Antibody; Anti-Periostin; C-terminal; anti-POSTN antibody

• Host: Rabbit
• Reactivity: Chicken, human, mouse
• Clonality: Polyclonal
• Specificity: Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry (data not shown). The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degree C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).
• Purity/Purification: Affinity Purified
• Form/Format: Liquid in PBS. Contains no preservative.
• Sequence Length: 810

• Applicable Applications for anti-POSTN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1,000; IHC: 1:100
• Antigen: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons.
• Immunogen Information: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons
• Immunogen Species: Mouse
• Reactivity Assumed Based on 100% Sequence Homology: Most mammalian and avian
• Species Reactivity Note: The antibody has been directly tested for reactivity in chicken, human, mouse and rat. The antibody is expected to work on all mammalian and avian species.
• Biological Significance: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008).
• Preparation and Storage: Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-POSTN antibody

Rabbit polyclonal antibody

References

• Kruzynska-Frejtag A, Wang J, Rogers R, Krug E, Hoffman S, Markwald RR, Conway SJ. (2004) Periostin is expressed within the developing teeth at the sites of epithelial-mesenchymal interaction. Develop Dynamics, 229:857-868.
• Norris RA, Borq TK, Butcher JT, Baudino TA, Banerjee I, Markwald RR (2008) Neonatal and adult cardiovascular pathophysiological remodeling and repair: developmental role of periostin. Ann NY Acad Sci. 1123:30-40.
• Norris RA, Damon B, Mironov V, Kasyanov V, Ramamurthi A, Moreno-Rodriguez R, Trusk T, Potts JD, Goodwin RL, Davis J, Hoffman S, Wen X, Sugi Y, Kern CB, Mjaatvedt CH, Turner DK, Oka T, Conway SJ, Molkentin JD, Forgacs G, Markwald RR. (2007) Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. J Cell Biochem. 101(3):695-711.

NCBI and Uniprot Product Information

• NCBI GI #: 311771596
• NCBI GeneID: 50706
• NCBI Accession #: NP_001185694.1
• NCBI GenBank Nucleotide #: NM_001198765.1
• UniProt Accession #: Q62009; Q8BMJ6; Q8K1K0
• Molecular Weight: 93
• NCBI Official Full Name: periostin isoform 2
• NCBI Official Synonym Full Names: periostin, osteoblast specific factor
• NCBI Official Symbol: Postn
• NCBI Official Synonym Symbols: PN; PLF; Osf2; peri; OSF-2; AI747096; A630052E07Rik
• NCBI Protein Information: periostin; periostin-like factor; osteoblast-specific factor 2; osteoblast specific factor 2 (fasciclin I-like)
• UniProt Protein Name: Periostin
• Protein Family: Periostin
• UniProt Gene Name: Postn
• UniProt Synonym Gene Names: Osf2; PN; OSF-2
• UniProt Entry Name: POSTN_MOUSE

NCBI Description

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

Function: Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. Ref.4 Ref.8

Subunit structure: Interacts with BMP1 and fibronectin. Ref.8

Subcellular location: Golgi apparatus. Secreted › extracellular space › extracellular matrix. Note: Colocalizes with BMP1 in the Golgi. Ref.4 Ref.8

Tissue specificity: Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. Ref.4 Ref.6

Developmental stage: In the heart, expressed from embryonic day 10.5. Continues to be strongly expressed throughout cardiac development and into adulthood. Ref.6

Induction: By TGF-beta. Ref.4

Post-translational modification: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Disruption phenotype: Reduced amount of collagen cross-linking in femur and periosteum. Ref.8

Sequence similarities: Contains 1 EMI domain.Contains 4 FAS1 domains.