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IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit DBT Polyclonal Antibody | anti-DBT antibody
DBT Polyclonal Antibody
Gene Names
DBT; E2; E2B; BCATE2; BCKADE2; BCKAD-E2
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
DBT, Antibody; DBT Polyclonal Antibody; E2; E2B; BCATE2; BCKADE2; BCKAD-E2; BCKDH-E2; BCOADC-E2; anti-DBT antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-DBT antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant fusion protein of human DBT
Preparation and Storage
Storage: -20 degree C/One year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A-431 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A-431 cells using DBT Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using DBT Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using DBT Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using DBT Polyclonal Antibody at 1:1000 dilution.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines using DBT Polyclonal Antibody at 1:1000 dilution.)
Related Product Information for anti-DBT antibody
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Product Categories/Family for anti-DBT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
482
NCBI Official Full Name
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
NCBI Official Synonym Full Names
dihydrolipoamide branched chain transacylase E2
NCBI Official Symbol
DBT
NCBI Official Synonym Symbols
E2; E2B; BCATE2; BCKADE2; BCKAD-E2
NCBI Protein Information
lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial; BCKAD E2 subunit; dihydrolipoyl transacylase; branched chain acyltransferase, E2 component; dihydrolipoyllysine-residue (2-methylpropanoyl)transfer
UniProt Protein Name
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
UniProt Gene Name
DBT
UniProt Entry Name
ODB2_HUMAN
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Product Notes
The DBT dbt (Catalog #AAA179260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DBT Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DBT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DBT dbt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DBT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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