Complement C1c+C1q ELISA Kit
Complement C1c+C1q ELISA Kit
Applications
ELISA
Synonyms
Complement C1c+C1q; Complement C1c+C1q ELISA Kit; C1c/C1q ELISA Kit; Complement C1c+C1q elisa kit
Applicable Applications for Complement C1c+C1q elisa kit
ELISA (EIA)
Application Notes
Highly purified human C1q is bound to microwells. Antibodies aginst this antigen, if present in diluted serum, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
Biological Significance
The presence of Anti-C1q autoantibodies is associated with several autoimmune and renal illnesses. Containing an occurrence of 100% in the hypocomplementaemic uricarial vasculitis syndrome (HUVS), Anti-C1q autoantibodies act as a marker for this disease. They were also described in systemic lupus erythemathodes (SLE) and especially in lupus nephritis.
Biohazard Information
All reagents of this test kit derived from human or animal serum or plasma have been tested and confirmed negative for HIV I/II, HbsAg and HCV. However, there is no test method that can offer complete assurance therefore all reagents, should be treated as potential biohazards in use and for disposal.
Preparation and Storage
Store at 4 degree C.
Related Product Information for Complement C1c+C1q elisa kit
ELISA kit for detection of Complement C1c and C1q in the research laboratory
Product Categories/Family for Complement C1c+C1q elisa kit
Similar Products
Product Notes
The Complement C1c+C1q (Catalog #AAA539749) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Complement C1c+C1q can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Highly purified human C1q is bound to microwells. Antibodies aginst this antigen, if present in diluted serum, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample. Researchers should empirically determine the suitability of the Complement C1c+C1q for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement C1c+C1q, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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