ApoB elisa kit
Human Apolipoprotein B ELISA Kit
Gene Names
LOC100523371; APOB
Synonyms
ApoB; Human Apolipoprotein B ELISA Kit; ApoB elisa kit
Sequence Length
3882
Preparation and Storage
Store at 4 degree C
Related Product Information for ApoB elisa kit
Apolipoprotein B (ApoB) is the primary protein constituent of low density lipoprotein, very-low density lipoprotein, and chylomicrons. ApoB directs cholesterol and triglyceride containing particles to tissues by ApoB receptor binding and internalization. The ratio of ApoA1 to Apolipoprotein B (ApoB), the primary component of low density lipoprotein, is an effective predictor of cardiovascular disease. The sensitive quantitative measurement of total human ApoB antigen in plasma, serum, urine and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. ApoB is present in human plasma and serum at a concentration of 0.5-1.25 mg/ml in adult males, 0.45-1.2 mg/ml in adult females, 0.11-0.31 mg/ml in newborns, and 0.23-1.39 mg/ml in children. The ratio of ApoA1/ApoB ranges from 0.85-2.24 in males and 0.76-3.23 in females. The assay measures total human ApoB in the 5-5000 ng/ml range. Samples giving human ApoB levels above 5000 ng/ml should be diluted in diluent before use. A 1:20,000 to 1:80,000 dilution for normal human plasma is suggested for best results. Human ApoB will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, monoclonal anti-human ApoB primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human ApoB. Color development is proportional to the concentration of total ApoB antigen in the samples. All reagents and standards are provided in these ELISA kits.
Product Categories/Family for ApoB elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
438,951 Da
NCBI Official Full Name
APOB
NCBI Official Symbol
LOC100523371
NCBI Official Synonym Symbols
APOB
NCBI Protein Information
apolipoprotein B-100
UniProt Protein Name
APOB
Protein Family
UniProt Gene Name
APOB
UniProt Entry Name
A0A0F6TNY5_PIG
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Product Notes
The ApoB apob (Catalog #AAA136010) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ApoB, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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