Human TAFI ELISA Kit | CPB2 elisa kit

Human TAFI Antigen ELISA Kit

Cat. Num. AAA136135

• Gene Names: CPB2; CPU; PCPB; TAFI
• Reactivity: Human
• Synonyms: TAFI; Human TAFI Antigen ELISA Kit; CPB2 elisa kit

• Reactivity: Human
• Sequence Length: 423

• Source: Multiple
• Epitope: Recognises TAFI and TAFIa
• Preparation and Storage: Store at 4 degree C; Expiration: 12 months from manufacture, see label for expiration date.; Shipping: Cold pack

Related Product Information for CPB2 elisa kit

Thrombin Activatable Fibrinolysis Inhibitor (TAFI), also known as carboxypeptidase B2 (PCB2), plasma carboxypeptidase-B (pCB), and carboxypeptidase-U (CPU), is a 401 amino acid 58 kDa plasma glycoprotein that is activated by thrombin. Activated TAFI (TAFIa) cleaves fibrin carboxy-terminal lysines reducing plasminogen and tPA binding sites resulting in downregulation of fibrinolysis. Although mice made genetically deficient for TAFI do not show a marked phenotype, change in human plasma TAFI level has been associated with many cardiovascular disorders. The sensitive quantitative measurement of total human TAFI antigen in plasma and serum is easily performed with this 96 well strip format ELISA kit. TAFI and TAFIa will be detected by the assay. The average concentration of TAFI in normal human plasma is 4.2 ug/ml. The assay measures total human TAFI in the 2-500 ng/ml range. Samples giving human TAFI levels above 500 ng/ml should be diluted in diluent before use. A 1:100 to 1:1,000 dilution for normal human plasma or serum is suggested for best results.
Human TAFI will bind to the affinity purified capture antibody coated on the microtiter plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human TAFI primary antibody binds to the captured protein. Excess primary antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human TAFI. Color development is proportional to the concentration of total TAFI antigen in the samples. All reagents and standards are provided in these ELISA kits.

Product Categories/Family for CPB2 elisa kit

ELISA kits; Plasma Proteins; Human Plasma Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 126273569
• NCBI GeneID: 1361
• NCBI Accession #: NP_001863.2
• NCBI GenBank Nucleotide #: NP_001863.2
• UniProt Accession #: Q96IY4
• NCBI Official Full Name: carboxypeptidase B2 preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B2
• NCBI Official Symbol: CPB2
• NCBI Official Synonym Symbols: CPU; PCPB; TAFI
• NCBI Protein Information: carboxypeptidase B2
• UniProt Protein Name: Carboxypeptidase B2
• Protein Family: Carboxypeptidase
• UniProt Gene Name: CPB2
• UniProt Synonym Gene Names: CPU; pCPB; TAFI
• UniProt Entry Name: CBPB2_HUMAN

NCBI Description

Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Protease; Secreted, signal peptide; EC 3.4.17.20

Chromosomal Location of Human Ortholog: 13q14.11

Cellular Component: extracellular space

Molecular Function: zinc ion binding; metallocarboxypeptidase activity

Biological Process: response to drug; fibrinolysis; negative regulation of fibrinolysis; response to heat; blood coagulation; proteolysis