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Typical Testing Data/Standard Curve (for reference only)

ApoAI ELISA Kit

Human ApoAI ELISA Kit

Synonyms
ApoAI; Human ApoAI ELISA Kit; ApoAI elisa kit
Ordering
For Research Use Only!
Samples
Plasma, serum or other biological fluid samples
Preparation and Storage
Upon receipt, aliquot and store the Human ApoAI Standard at -20 degree C to avoid multiple freeze/thaw cycles. Store all other components at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ApoAI elisa kit
Background: Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Plasma apolipoproteins can be grouped into two classes: the nonexchangeable apolipoproteins (ApoB-100 and ApoB-48), and the exchangeable apolipoproteins (ApoAI, ApoAII, ApoAIV, ApoCI, ApoCII, ApoCIII, and ApoE). ApoAI comprises approximately 70% of the protein moiety in HDL (Figure 1). It is a single polypeptide chain consisting of 223 amino acid residues without disulfide bond and with aspartic acid as the the N-terminal residue and glutamic acid as the C-terminal residue. It has an approximate molecular weight 28 kDa. ApoAI activates lecithin-chlesterol (LCAT) acyltransferase, which is responsible for cholesterol esterification in plasma. Human ApoAI ELISA Kit is an enzyme immunoassay developed for the detection and quantitation of human ApoAI in plasma, serum or other biological fluid samples. The kit has detection sensitivity limit of 50 pg/mL human ApoAI. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.
References
1. Segrest J. P., Garber D. W., Brouillette C. G., Harvey S. C., and Anantharamaiah G. M. Adv. Protein Chem. 45: 303-369, 1994.
2. Segrest J. P., Jones M. K., De Loof H., Brouillette C. G., Venkatachalapathi Y. V., and Anantharamaiah G. M. J. Lipid Res. 33: 141-166, 1992.
3. Vaisar, T., Pennathur, S., Green, P. S., Gharib, S. A., Hoofnagle, A. N., Cheung, M. C., Byun, J., Vuletic, S., Kassim, S., Singh, P., Chea, H., Knopp, R. H., Brunzell, J., Geary, R., Chait, A., Zhao, X. Q., Elkon, K., Marcovina, S., Ridker, P., Oram, J. F., and Heinecke, J. W. J. Clin. Investig. 117, 746-756, 2007.
4. Tailleux, A., Duriez, P., Fruchart, J. C., and Clavey, V. Atherosclerosis 164, 1-13, 2003.

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Product Notes

The ApoAI (Catalog #AAA168533) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "ApoAI, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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