Related Product Information for EL elisa kit
Principle of the Assay: EL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EL antibody and an EL-HRP conjugate. The assay sample and buffer are incubated together with EL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EL concentration since EL from samples and EL-HRP conjugate compete for the anti-EL antibody binding site. Since the number of sites is limited, as more sites are occupied by EL from the sample, fewer sites are left to bind EL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EL concentration in each sample is interpolated from this standard curve.
Product Categories/Family for EL elisa kit
NCBI Description
The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]
Uniprot Description
LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - glycerolipid; Phospholipase; Secreted, signal peptide; Secreted; EC 3.1.1.3
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome
Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity
Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis
Product Notes
The Sheep EL lipg (Catalog #
AAA742140) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA742140 ELISA Kit recognizes Sheep EL.
It is sometimes possible for the material contained within the vial of
"Endothelial lipase, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
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aspect of this datasheet at
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