Sheep Endothelial lipase ELISA Kit | EL elisa kit

Sheep Endothelial lipase ELISA Kit

Cat. Num. AAA742140

• Gene Names: LIPG; EL; EDL; PRO719
• Reactivity: Sheep
• Synonyms: Endothelial lipase; Sheep Endothelial lipase ELISA Kit; EL elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This EL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep EL. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EL elisa kit

Principle of the Assay: EL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-EL antibody and an EL-HRP conjugate. The assay sample and buffer are incubated together with EL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the EL concentration since EL from samples and EL-HRP conjugate compete for the anti-EL antibody binding site. Since the number of sites is limited, as more sites are occupied by EL from the sample, fewer sites are left to bind EL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EL concentration in each sample is interpolated from this standard curve.

Product Categories/Family for EL elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 5174497
• NCBI GeneID: 9388
• NCBI Accession #: NP_006024.1
• NCBI GenBank Nucleotide #: NM_006033.2
• Molecular Weight: 39,362 Da
• NCBI Official Full Name: endothelial lipase
• NCBI Official Synonym Full Names: lipase, endothelial
• NCBI Official Symbol: LIPG
• NCBI Official Synonym Symbols: EL; EDL; PRO719
• NCBI Protein Information: endothelial lipase; lipoprotein lipase H; endothelial cell-derived lipase
• UniProt Protein Name: Endothelial lipase
• Protein Family: Endothelial lipase
• UniProt Gene Name: LIPG
• UniProt Synonym Gene Names: UNQ387/PRO719; EDL; EL
• UniProt Entry Name: LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Phospholipase; Secreted, signal peptide; Secreted; EC 3.1.1.3

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis