SDS-Page
eIF5A recombinant protein
eIF5A, 1-154aa, Human, E Coli
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
eIF5A; 1-154aa; Human; E Coli; Eukaryotic translation initiation factor 5A isoform B; EIF-5A; EIF5A1; eIF5AI; Eukaryotic translation initiation factor 5A isoform B eIF 4D; EIF 5A; eIF 5A 1; eIF 5A1; eIF4D; uORF; uORF A; Eukaryotic initiation factor 5A; Rev binding factor; Eukaryotic initiation factor 5A isoform 1; MGC99547; Eukaryotic translation initiation factor 5A; Eukaryotic translation initiation factor 5A 1; MGC104255.; eIF5A recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 50 mM Tris-HCl Buffer pH 7.5 containing 10 % glycerol
Concentration
1.0 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK
Sequence Length
154
Applicable Applications for eIF5A recombinant protein
SDS-PAGE
Antigen Species
Human
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for eIF5A recombinant protein
Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer. Recombinant human eIF5A protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Categories/Family for eIF5A recombinant protein
References
Saini P, et al. (2009). Nature. 459 (7243):118-21; Gosslau A., et al. (2009). J Cell Physiol. 219 (2):485-93;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16.8 kDa (154 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
eukaryotic translation initiation factor 5A-1 isoform B
UniProt Protein Name
Eukaryotic translation initiation factor 5A-1
Protein Family
UniProt Gene Name
EIF5A
UniProt Synonym Gene Names
eIF-5A-1; eIF-5A1; eIF-5A
Uniprot Description
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. With syntenin SDCBP, functions as a regulator of p53/TP53 and p53/TP53-dependent apoptosis. Regulates also TNF-alpha-mediated apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation. Also described as a cellular cofactor of human T-cell leukemia virus type I (HTLV-1) Rex protein and of human immunodeficiency virus type 1 (HIV-1) Rev protein, essential for mRNA export of retroviral transcripts.
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Product Notes
The eIF5A eif5a (Catalog #AAA203210) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's eIF5A can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the eIF5A eif5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MADDLDFETG DAGASATFPM QCSALRKNGF VVLKGRPCKI VEMSTSKTGK HGHAKVHLVG IDIFTGKKYE DICPSTHNMD VPNIKRNDFQ LIGIQDGYLS LLQDSGEVRE DLRLPEGDLG KEIEQKYDCG EEILITVLSA MTEEAAVAIK AMAK. It is sometimes possible for the material contained within the vial of "eIF5A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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