Mouse anti-Human VEGF Monoclonal Antibody | anti-VEGF antibody

Mouse anti Human VEGF:Biotin

Cat. Num. AAA226247

• Reactivity: Human
• Applications: ELISA
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: VEGF; Monoclonal Antibody; Mouse anti Human VEGF:Biotin; VEGF antibody | CD19-4G11; anti-VEGF antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CD19-4G11
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Phosphate buffered saline; 0.09% Sodium Azide (NaN3); 1% Bovine Serum Albumin; Purified IgG conjugated to Biotin - liquid
• Concentration: IgG concentration 0.1mg/ml (varies by lot)

• Applicable Applications for anti-VEGF antibody: ELISA (EIA)
• Application Notes: ELISA: 1.0ug/ml
• Immunogen: E Coli derived recombinant full length human VEGF (Ala27-Arg147)
• Fusion Partners: Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
• Conjugation: Biotin
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.; ; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

ELISA (EIA)

(Sandwich ELISA analysis of human VEGF using Mouse anti Human VEGF as a capture reagent and biotinylated Mouse anti Human VEGF (MBS226247) as a detection reagent with purified recombinant VEGF as the antigen. Detection was performed using HRP co)

Related Product Information for anti-VEGF antibody

Mouse anti Human VEGF antibody, clone CD19-4G11, recognizes vascular endothelial growth factor alpha (VEGF-A) which forms a homodimer and is a potent stimulator of angiogenesis of both normal and cancerous cells. It acts as a regulator of vasculogenesis, angiogenesis and endothelial cell growth. VEGF-A is secreted by many different cell types such as endothelial cells (Nissen et al. 1998), smooth muscle cells (Brogi et al. 1994), neutrophils (Gaudry et al. 1997), platelets (Banks et al. 1998), macrophages and 60% of all tumors (Berse et al. 1992).

The biotinylated Mouse anti Human VEGF antibody, clone CD19-4G11 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human VEGF antibody, clone CD17-1E1 as the capture antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 190886449
• NCBI GeneID: 85480
• NCBI Accession #: NP_612561.2
• NCBI GenBank Nucleotide #: NM_138551.4
• UniProt Accession #: Q969D9; Q8IW99
• Molecular Weight: 18,141 Da
• NCBI Official Full Name: thymic stromal lymphopoietin isoform 2
• NCBI Official Synonym Full Names: thymic stromal lymphopoietin
• NCBI Official Symbol: TSLP
• NCBI Protein Information: thymic stromal lymphopoietin
• UniProt Protein Name: Thymic stromal lymphopoietin
• Protein Family: VEGF coregulated chemokine
• UniProt Gene Name: TSLP
• UniProt Entry Name: TSLP_HUMAN

NCBI Description

This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

TSLP: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q22.1

Cellular Component: extracellular space

Molecular Function: cytokine activity