Human Eukaryotic Translation Initiation Factor 3A ELISA Kit | EIF3A elisa kit

Human Eukaryotic Translation Initiation Factor 3A ELISA Kit

Cat. Num. AAA39236

• Gene Names: eif3s10; zgc:66194; zgc:77263; wu:fi15c03
• Reactivity: Human
• Synonyms: Eukaryotic Translation Initiation Factor 3A; Human Eukaryotic Translation Initiation Factor 3A ELISA Kit; EIF3A elisa kit

• Reactivity: Human
• Specificity: This kit recognizes recombinant and natural Human EIF3A, no significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 3.12 - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Human EIF3A in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Human EIF3A concentration. The concentration of Human EIF3A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EIF3A elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 45433533
• NCBI GeneID: 327515
• NCBI Accession #: NP_956114.2
• NCBI GenBank Nucleotide #: NM_199820.2
• UniProt Accession #: Q6PCR7; Q6NYB1
• Molecular Weight: 151,292 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 3 subunit A
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 3, subunit 10 (theta)
• NCBI Official Symbol: eif3s10
• NCBI Official Synonym Symbols: zgc:66194; zgc:77263; wu:fi15c03
• NCBI Protein Information: eukaryotic translation initiation factor 3 subunit A; eIF3a; eIF-3-theta; eukaryotic translation initiation factor 3 subunit 10
• UniProt Protein Name: Eukaryotic translation initiation factor 3 subunit A
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: eif3a
• UniProt Synonym Gene Names: eif3s10; eIF3a
• UniProt Entry Name: EIF3A_DANRE

Uniprot Description

Function: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome

By similarity. HAMAP-Rule MF_03000

Subunit structure: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: eif3a, eif3b, eif3c, eif3d, eif3e, eif3f, eif3g, eif3h, eif3i, eif3j, eif3k, eif3l and eif3m

By similarity.

Subcellular location: Cytoplasm

By similarity HAMAP-Rule MF_03000.

Sequence similarities: Belongs to the eIF-3 subunit A family.Contains 1 PCI domain.