Human eukaryotic translation initiation factor 2C, 1 ELISA Kit | EIF2C1 elisa kit

Human Protein argonaute-1, EIF2C1 ELISA Kit

Cat. Num. AAA9340176

• Gene Names: AGO1; Q99; EIF2C; EIF2C1; GERP95
• Reactivity: Human
• Synonyms: eukaryotic translation initiation factor 2C; 1; Human Protein argonaute-1; EIF2C1 ELISA Kit; Human Protein argonaute-1 (EIF2C1) ELISA kit; AGO1; DKFZp686M13167; EIF2C; GERP95; Q99; Golgi Endoplasmic Reticulum protein 95 kDa; argonaute 1; putative RNA-binding protein Q99; EIF2C1 elisa kit

• Reactivity: Human

• Samples: Undiluted original Human serum, plasma or tissue homogenates samples
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12ng/ml-100ng/ml
• Sensitivity: 1.0ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for EIF2C1 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only! This kit is intended to be used for determine the level of EIF2C1 (hereafter termed "analyte") in undiluted original Human serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 6912352
• NCBI GeneID: 26523
• NCBI Accession #: NP_036331.1
• NCBI GenBank Nucleotide #: NM_012199.2
• UniProt Accession #: Q9UL18; Q5TA57; Q6P4S0
• Molecular Weight: 97,214 Da
• NCBI Official Full Name: protein argonaute-1
• NCBI Official Synonym Full Names: argonaute RISC catalytic component 1
• NCBI Official Symbol: AGO1
• NCBI Official Synonym Symbols: Q99; EIF2C; EIF2C1; GERP95
• NCBI Protein Information: protein argonaute-1; hAgo1; eIF2C 1; eIF-2C 1; argonaute1; argonaute 1; putative RNA-binding protein Q99; Golgi Endoplasmic Reticulum protein 95 kDa; eukaryotic translation initiation factor 2C, 1
• UniProt Protein Name: Protein argonaute-1
• UniProt Gene Name: AGO1
• UniProt Synonym Gene Names: EIF2C1; Argonaute1; hAgo1; eIF-2C 1; eIF2C 1
• UniProt Entry Name: AGO1_HUMAN

NCBI Description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4. [provided by RefSeq, Jul 2008]

Uniprot Description

AGO1: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA. Belongs to the argonaute family. Ago subfamily.

Protein type: Translation; RNA-binding

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; polysome; cytoplasm; ribonucleoprotein complex; cytosol

Molecular Function: endoribonuclease activity; protein binding; miRNA binding; RNA binding

Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; nerve growth factor receptor signaling pathway; miRNA-mediated gene silencing, mRNA cleavage; innate immune response; miRNA-mediated gene silencing, negative regulation of translation; positive regulation of transcription from RNA polymerase II promoter; gene expression; posttranscriptional gene silencing