Immunofluorescence (IF)
(MBS9604768 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse DGKD Polyclonal Antibody | anti-DGKD antibody
DGKD Antibody
Gene Names
DGKD; dgkd-2; DGKdelta
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DGKD; Polyclonal Antibody; DGKD Antibody; 130 kDa diacylglycerol kinase; DAG kinase delta; DGK delta; DGK-delta; DGKD_HUMAN; DGKdelta; Diacylglycerol kinase delta; Diglyceride kinase delta; FLJ26930; KIAA0145; anti-DGKD antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DGKD antibody detects endogenous levels of total DGKD
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1170
Applicable Applications for anti-DGKD antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Tissue Specificity
Isoform 2 is ubiquitously expressed also in tumor tissues. Isoform 1 is expressed in ovary, spleen and some tumor-derived cells.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (90%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9604768 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9604768 staining HuvEc cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using DGKD antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using DGKD antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using DGKD antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse brain, using DGKD antibody.)
Related Product Information for anti-DGKD antibody
Function: May function as signaling molecule.
Subunit Structure: The two isoforms are able to form homo-and hetero-oligomer structures (at least tetramers). Isoform 2 interacts with AP2A2.
Post-translational Modifications: Isoform 1 H domain is phosphorylated.
Similarity: Belongs to the eukaryotic diacylglycerol kinase family.
Subunit Structure: The two isoforms are able to form homo-and hetero-oligomer structures (at least tetramers). Isoform 2 interacts with AP2A2.
Post-translational Modifications: Isoform 1 H domain is phosphorylated.
Similarity: Belongs to the eukaryotic diacylglycerol kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 135 kD
Predicted: 135 kDa
Predicted: 135 kDa
NCBI Official Full Name
diacylglycerol kinase delta isoform 1
NCBI Official Synonym Full Names
diacylglycerol kinase delta
NCBI Official Symbol
DGKD
NCBI Official Synonym Symbols
dgkd-2; DGKdelta
NCBI Protein Information
diacylglycerol kinase delta
UniProt Protein Name
Diacylglycerol kinase delta
Protein Family
UniProt Gene Name
DGKD
UniProt Synonym Gene Names
KIAA0145; DAG kinase delta; DGK-delta
NCBI Description
This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
May function as signaling molecule.
Research Articles on DGKD
Similar Products
Product Notes
The DGKD dgkd (Catalog #AAA9604768) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DGKD Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DGKD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the DGKD dgkd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
