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Gene Sequencing (extract)

Early Growth Response Protein 1 (EGR1) Recombinant Protein | EGR1 recombinant protein

Recombinant Early Growth Response Protein 1 (EGR1)

Gene Names
Egr1; egr; TIS8; Zenk; Egr-1; NGFIA; Zfp-6; ETR103; Krox-1; Krox24; NGF1-A; NGFI-A; Zif268; Krox-24; A530045N19Rik
Reactivity
Mus musculus (Mouse)
Applications
SDS-Page, Western Blot
Purity
> 97%
Synonyms
Early Growth Response Protein 1 (EGR1); Recombinant Early Growth Response Protein 1 (EGR1); EGR1 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 97%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 450ug/mL (varies by lot)
Sequence
QGLENRTQ QPSLTPLSTI KAFATQSGSQ DLKALNTTYQ SQLIKPSRMR KYPNRPSKTP PHERPYACPV ESCDRRFSRS DELTRHIRIH TGQKPFQCRI CMRNFSRSDH LTTHIRTHTG EKPFACDICG RKFARSDERKRHTKIHLRQK DKKADKSVVA S
Applicable Applications for EGR1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Gln273~Ser431
Tags
N-terminal His Tag
Subcellular Location
Nucleus
Traits
Freeze-dried powder
Predicted isoelectric point
10.5
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.

Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Gene Sequencing (extract)

Gene Sequencing (extract)

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 22.2kDa
Accurate Molecular Mass: 22&27kDa as determined by SDS-PAGE reducing conditions
NCBI Official Full Name
early growth response protein 1
NCBI Official Synonym Full Names
early growth response 1
NCBI Official Symbol
Egr1
NCBI Official Synonym Symbols
egr; TIS8; Zenk; Egr-1; NGFIA; Zfp-6; ETR103; Krox-1; Krox24; NGF1-A; NGFI-A; Zif268; Krox-24; A530045N19Rik
NCBI Protein Information
early growth response protein 1; Krox-24 nuclear protein; transcription factor Zif268; zinc finger protein Krox-24; nerve growth factor-induced protein A
UniProt Protein Name
Early growth response protein 1
UniProt Gene Name
Egr1
UniProt Synonym Gene Names
Egr-1; Krox-24; EGR-1; NGFI-A
UniProt Entry Name
EGR1_MOUSE

Uniprot Description

EGR1: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation. By growth factors. Belongs to the EGR C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding; Transcription factor

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; DNA binding; nucleic acid binding; sequence-specific DNA binding; metal ion binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: regulation of long-term neuronal synaptic plasticity; transcription from RNA polymerase II promoter; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; response to insulin stimulus; BMP signaling pathway; regulation of apoptosis; learning and/or memory; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; response to glucose stimulus; positive regulation of transcription from RNA polymerase II promoter; regulation of protein sumoylation; negative regulation of apoptosis; T cell differentiation

Research Articles on EGR1

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Product Notes

The EGR1 egr1 (Catalog #AAA2018279) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Early Growth Response Protein 1 (EGR1) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Early Growth Response Protein 1 (EGR1) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the EGR1 egr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QGLENRTQ QPSLTPLSTI KAFATQSGSQ DLKALNTTYQ SQLIKPSRMR KYPNRPSKTP PHERPYACPV ESCDRRFSRS DELTRHIRIH TGQKPFQCRI CMRNFSRSDH LTTHIRTHTG EKPFACDICG RKFARSDERK RHTKIHLRQK DKKADKSVVA S. It is sometimes possible for the material contained within the vial of "Early Growth Response Protein 1 (EGR1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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