Human epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) ELISA Kit | EGFR elisa kit

Human epidermal growth factor receptor, EGFR ELISA Kit

Cat. Num. AAA701531

• Gene Names: EGFR; c-erbB
• Reactivity: Human
• Synonyms: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian); Human epidermal growth factor receptor; EGFR ELISA Kit; ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor recept; EGFR; EGFR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human EGFR. No significant cross-reactivity or interference between human EGFR and analogues was observed.
• Sequence Length: 1226

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.312 ng/ml -20 ng/ml.
• Sensitivity: The minimum detectable dose of human EGFR is typically less than 0.019 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EGFR elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EGFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 158261996
• NCBI GeneID: 396494
• NCBI Accession #: NP_990828.2
• NCBI GenBank Nucleotide #: NM_205497.2
• UniProt Accession #: P13387
• Molecular Weight: 77,427 Da
• NCBI Official Full Name: epidermal growth factor receptor
• NCBI Official Synonym Full Names: epidermal growth factor receptor
• NCBI Official Symbol: EGFR
• NCBI Official Synonym Symbols: c-erbB
• NCBI Protein Information: epidermal growth factor receptor; CER; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• UniProt Protein Name: Epidermal growth factor receptor
• Protein Family: Epidermal growth factor receptor
• UniProt Gene Name: EGFR
• UniProt Synonym Gene Names: CER
• UniProt Entry Name: EGFR_CHICK

Uniprot Description

Function: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade.

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme regulation: Endocytosis and inhibition of the activated EGFR by phosphatases constitute immediate regulatory mechanisms. Moreover, inducible feedback inhibitors may constitute alternative regulatory mechanisms for the EGFR signaling.