EED recombinant protein

EED Protein, Human, Recombinant (His & GST Tag)

Cat. Num. AAA8120783

• Gene Names: EED; HEED; WAIT1
• Purity: >93% as determined by SDS-PAGE
• Synonyms: EED; EED Protein; Human; Recombinant (His & GST Tag); Human EED/Embryonic Ectoderm Development Protein (His & GST Tag); HEED Protein; WAIT1 Protein; embryonic ectoderm development; EED recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >93% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 2mM GSH, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg441

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the human EED (O75530-1)(Met 1-Arg 441) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for EED recombinant protein

Background: EED is essential for regulating the repressive histone modification, histone 3 lysine 27 tri-methylation (H3K27me3) at many developmental genes. EED depletion significantly impeded erythroid maturation. EED depletion impaired the generation of hematopoietic stem cells. The function of EED within polycomb repressive complex 2 (PRC2) is mediated by a complex network of protein-protein interactions. The targeted disruption of EED's reader function can lead to allosteric inhibition of PRC2 catalytic activity. Eed(Delta/Delta) HSPCs exhibited increased attachment to a major extracellular matrix component, fibronectin. Thus, EED deficiency increases proliferation on one side but promotes quiescence possibly by enhanced adhesion to the hematopoietic niche on the other, and these conflicting events would lead to abnormal differentiation and functional defect of Eed(Delta/Delta) HSPCs.

References

Ura H, et al. (2011) Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation. EMBO J. 30(11): 2190-204.Montgomery ND, et al. (2007) Molecular and functional mapping of EED motifs required for PRC2-dependent histone methylation. J Mol Biol. 374(5): 1145-57.Jin Q, et al. (2003) The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor. J Biol Chem. 278(33): 30677-85.Showell C, et al. (2002) Identification of putative interaction partners for the Xenopus Polycomb-group protein Xeed. Gene. 291(1-2): 95-104.Rinchik EM, et al. (1993) N-ethyl-N-nitrosourea-induced prenatally lethal mutations define at least two complementation groups within the embryonic ectoderm development (eed) locus in mouse chromosome 7. Mamm Genome. 4(7): 349-53.

NCBI and Uniprot Product Information

• NCBI GI #: 24041020
• NCBI GeneID: 8726
• NCBI Accession #: NP_003788.2
• NCBI GenBank Nucleotide #: NM_003797.3
• UniProt Accession #: O75530; O00149; Q6NTH2; Q7LDA5; Q7LDG8; Q86VV2; Q9UNY7; A8K7V5
• Molecular Weight: 45,524 Da
• NCBI Official Full Name: polycomb protein EED isoform a
• NCBI Official Synonym Full Names: embryonic ectoderm development
• NCBI Official Symbol: EED
• NCBI Official Synonym Symbols: HEED; WAIT1
• NCBI Protein Information: polycomb protein EED; WD protein associating with integrin cytoplasmic tails 1
• UniProt Protein Name: Polycomb protein EED
• Protein Family: Polycomb protein
• UniProt Gene Name: EED
• UniProt Synonym Gene Names: hEED; WAIT-1
• UniProt Entry Name: EED_HUMAN

NCBI Description

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EED: a member of the superfamily of Polycomb group (PcG) methyltransferase proteins with WD-40 repeats. PcG complexes mediate chromatin modifications that contribute to transcriptional silencing during development and carcinogenesis. A part of the Polycomb-repressive complex 2 (PRC2) along with EZH2, SUZ12, RBBP4 and RBBP7 and possibly AEBP2. Interacts with HDAC, HDAC2, histone H1 and YY1. PRC2 trimethylates histone H3 on K9 and K27, transcriptionally repressing target gene including HOXC8, HOXA9, MYT1 and CDKN2A. Appears to be overexpressed in breast and colon cancers. Inhibitors of PRC2-mediated gene repression are being investigated as a cancer therapy. EED and EZH2 are also implicated in the silencing of the inactive X chromosome. Three human isoforms produced by alternative splicing or initiation have been described, and additional isoforms may be produced by alternative initiation.

Protein type: Methyltransferase

Chromosomal Location of Human Ortholog: 11q14.2-q22.3

Cellular Component: nucleoplasm; sex chromatin; pronucleus; ESC/E(Z) complex; cytoplasm; nucleolus; nucleus

Molecular Function: histone methyltransferase activity; identical protein binding; protein binding; chromatin binding

Biological Process: histone methylation; genetic imprinting; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; negative regulation of transcription, DNA-dependent; regulation of gene expression, epigenetic