Mouse Endothelin-3 ELISA Kit | Edn3 elisa kit

Mouse Endothelin-3 ELISA Kit

Cat. Num. AAA762562

• Gene Names: EDN3; ET3; ET-3; WS4B; HSCR4; PPET3
• Reactivity: Mouse
• Synonyms: Endothelin-3; Mouse Endothelin-3 ELISA Kit; Edn3/EDN3/endothelin 3/endothelin-3/ET3/ET-3/HSCR4/PPET3/preproendothelin-3/Preproendothelin-3/truncated endothelin 3/WS4B; Edn3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Edn3. No significant cross-reactivity or interference between Edn3 and analogues was observed.
• Sequence Length: 198

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Edn3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 698848571
• NCBI GeneID: 1908
• NCBI Accession #: NP_001289384.1
• NCBI GenBank Nucleotide #: NM_001302455.1
• Molecular Weight: 23,596 Da
• NCBI Official Full Name: endothelin-3 isoform 4 preproprotein
• NCBI Official Synonym Full Names: endothelin 3
• NCBI Official Symbol: EDN3
• NCBI Official Synonym Symbols: ET3; ET-3; WS4B; HSCR4; PPET3
• NCBI Protein Information: endothelin-3
• UniProt Protein Name: Endothelin-3
• Protein Family: Endothelin
• UniProt Gene Name: EDN3
• UniProt Synonym Gene Names: ET-3; PPET3
• UniProt Entry Name: EDN3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Altered expression of this gene is implicated in tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

EDN3: Endothelins are endothelium-derived vasoconstrictor peptides. Defects in EDN3 are the cause of Hirschsprung disease type 4 (HSCR4); also known as aganglionic megacolon (MGC). A genetic disorder of neural crest development characterized by the absence of intramural ganglion cells in the hindgut; often resulting in intestinal obstruction. Defects in EDN3 are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Defects in EDN3 are a cause of Waardenburg syndrome type 4 (WS4B); also known as Waardenburg-Shah syndrome. WS4B is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease). Belongs to the endothelin/sarafotoxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.2-q13.3

Cellular Component: cell; extracellular region; extracellular space; intracellular

Molecular Function: endothelin B receptor binding; hormone activity; receptor binding

Biological Process: artery smooth muscle contraction; blood circulation; cell surface receptor linked signal transduction; cell-cell signaling; cellular calcium ion homeostasis; inositol phosphate-mediated signaling; melanocyte differentiation; multicellular organismal development; neural crest cell migration; neuron differentiation; neutrophil chemotaxis; peptide hormone secretion; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of heart rate; positive regulation of hormone secretion; positive regulation of leukocyte chemotaxis; positive regulation of MAP kinase activity; positive regulation of mitosis; regulation of gene expression; regulation of pigmentation during development; regulation of systemic arterial blood pressure by endothelin; regulation of vasoconstriction; signal transduction; vasoconstriction; vein smooth muscle contraction

Disease: Central Hypoventilation Syndrome, Congenital; Hirschsprung Disease, Susceptibility To, 4; Waardenburg Syndrome, Type 4b